| Zanthoxylum bungeanum is deciduous shrubs or small trees of Rutaceae Zanthoxylum L. It is an important seasoning and medicinal tree species and has a long history of cultivation in China. Since the reform and opening-up with people living standard of continual improve, the demand of zanthoxylum as mixtion article become more and more large and new utilization approach of zanthoxylum is developmented, for example, zanthoxylum bud vegetable , zanthoxylum perfume, zanthoxylum volatile-oil, Using zanthoxylum production insecticide and extraction biological wood oil, and so on. These cause prices continual increase, the market price of high quality zanthoxylum arrive at 56 yuan / kg. in 2006. In recent years, the area of cultivation of Zanthoxylum have been expanding and the output improve substantially, it made many farmers get rich. In some places, Zanthoxylum industry has even become a important industry of local economies. Zanthoxylum breeding is relatively long-term backward level, hasn't been far from meeting the requirements of the current development trend. This paper conduct an investigation and collection for the Zanthoxylum germplasm resources, it is ready for future breeding and preparing materials; Tissue Culture Research of Zanthoxylum bungeanum regeneration system is for improving backward Zanthoxylum breeding and a poor means; Research of micro-cutting technology is ready for seeding breeding and promotion. The main results are as follows:1, Germplasm collectionAfter two-years investigation and collection, there is more clear understanding the distribution of Zanthoxylum in northern China, the scale of cultivation around the major varieties, primitive and cultivated species and wild relatives; there is a detailed research on biological characteristics, as well as the economic value, breeding value of the scale of cultivation around the major varieties, primitive and early cultivars and species of wild relatives,it has accumulated valuable materials for Zanthoxylum research in the future. 44 species or strains are collected from main cultivation in northern China, including 26 major cultivars or strains, 7 original cultivars, 11 wild relatives and a plus tree. In nursery of Forestry College of Northwest Agriculture and Forestry University of Science and Technology established germplasm resources for nursery work.Germplasm collected from the various resources have been a certain number of seedling as five repeat of complete randomized block design. It provided the conditions for categories of the Zanthoxylum, evaluation and the cultivation of new varieties, at the same time it provided an important value for the protection of the diversity of germplasm resources, the preservation of germplasm resources, utilization and development。2. The research of regeneration system of Zanthoxylum tissue cultureBy using the soft stem, tender leaves and phloem of Zanthoxvlum in Fengxian county for the explants, the test study different types and concentrations of cytokinin (TDZ, ZT and 6-BA) and growth hormone (IBA and NAA) portfolio on the impact of all aspects of regeneration system of Zanthoxylum tissue culture and establish the regeneration system of Zanthoxylum tissue culture. Test results show that tender leaves is the best material that induced callus of Zanthoxylum, the best medium for induction callus is MS+0.5mg/L 2,4-D+0.5 mg/L BA; induction rate is 90%, and callus grow well; With hormone combination and density 0.03 mg/L TDZ+0.1 mg/LBA, could induce the callus to differentiation the normal adventitious buds, induction rate is 70%.Different light intensity, the callus induced by different materials, as well as the number of different subculture callus differentiation ability are significant difference; the best medium for endosperm-derived is MS+0.4 mg/L 6-BA+ 0.3mg/L IBA; the coefficient of propagation is 20; the best medium for root induction was 1/4MS+0.4 mg/L IBA, the rooting rate was more than 90%.Zanthoxylum cutting of plantlets bottles reasults show that as the sand and perlite ratio is 1 and 1and is the ideal cutting vitro matrix. The effect of 100～500ppmIBA concentration on the cutting is little, 300 ppm is superior to other it has an important impact on cutting moisturizing and disinfecting matrix。The regenerated system is established. Zanthoxylum breeding in the future has opened up a new way and is very great significance.3. The research of cutting of micro-technology of ZanthoxylumThis test include the impact on different substrates, different treatment and different concentrations of IBA processing time on the one-year-old shoots Zanthoxylum rooting cuttings. The results show that the effect of different matrix on the rooting rate and the number of Zanthoxylum cuttings is clear, the best matrix is the sand and perlite ratio is 1 and 1, the effect is better than that of Vermiculite matrix in highest water content; the effect of different IBA on rooting is clear, the best effect is 100 ppm, higher than 150 ppm IBA deal easily lead to callus by cuttings; different processing time have a great influence on rooting rate, the best time is 24 hours, length of water loss due to leaf cuttings rooting rate is much lower. If disinfection in the matrix is full, the ratio of perlite and sand is 1and1, cuttings with 100 ppm IBA for 24 hours, the rooting rate will reach 100%,the average number of rooting is 6~8.
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