Isolation And Identification Of Dairy Cow Endometritis Pathogenic Bacteria And Bacteriostatic Experiment In Beijing Region

Cow endometritis is a common postpartum obstetric disease mainly caused by bacteria infections, which can result in reproductive obstacles and great economic loss in the dairy industry.The uterie secretions of 8 postpartum healthy cows and 60 endometritis cows from Beijing region were collected, and the pathogenic bacteria were isolated and identificated. The bacteria in 0-30 d postpartum healthy cow uterus were isolated, the bacteria number was the most in 2-7d postpartum. The main pathogenic bacteria of endometritis cows were Escherichia coli, Staphylococcus, Streptococus agalactiae, Streptococus uberis, Enterococcus faecalis, Bacillus respectively. It provided a reference for treatment of cow endometritis.In order to research the sensitive degree of the herbal medicine and the western drugs on cow endometritis pathogenic bacteria, the antibiotics and herbal medicine were used for in vitro bacteriostatic experiment of the main pathogenic bacteria in endometritis cows. The results showed that the bacteriostatic effects of Ampicillin and Ciprofloxacin were better, but those of Penicillin and Streptomycin were less. All of them showed drug resistance. The bacteriostatic effects of herbal medicine Honeysuckle, Forsythia, Taraxacum were better. It provided important basis for treatment of cow endometriosis in clinic.The bacteriostatic effects of the herbal medicine were determined by 2-fold dilution method. The results showed that the herbal medicine prescription composed of Honeysuckle, Forsythia, Taraxacum etc had remarkable bacteriostatic effects for cow endometritis pathogenic bacteria. By bacteriostatic experiment in vitro, the herbal formula could be selected for dairy cow endometritis treatment.In vivo experiment, the blood samples from 10 healthy dairy cows and 20 endometritis dairy cows before and after drug treatment were collected. SOD and MDA etc were measured by clinical pharmacodynamics test, to observe the clinical effect of the herbal formula on cow endometritis. The results showed this herbal formula could decrease the content of MDA, increase SOD and GSH-Px activities, enhance the contents of IgG and IgA, and improve the immunity of endometritis dairy cows.Conclusion:The antibiotics had some bacteriostatic effects to the endometritis pathogenic bacteria, but showed drug resistance. The herbal formula showed better effect for treatment of cow endometriosis in clinic.