Isolation And Identification Of Bacteria From Uterine Of Sows Suffered From Endometritis

PURPOSE: In order to find out the bacteria category, to discuss pathogenesis of sow's endometritis, and to provide scientific method of control and treatment of sow's endometritis, Bacteria groups of sow's uterine suffered with endometritis were isolated and identified and antibiotic susceptibility of the pathogenic bacteria were examined.MEHTOUD: Sows were butchered in slaughter house. 15 sows with non-endometritis and 45 sows with endometritis were selected for this study. (1) Tissue samples of endometritis were collected from uterus, tissue slices were made and stained by HE, and pathological changes were observed under microscope. (2) Fresh mucus samples of uterine horn and uterine cervix were collected from the sows of non-endometritis and endometritis, inoculated into the culture medium of liquid broths and cultured for 24～48 h in 37℃. And then the liquid cultures were transferred to and inoculated on solid culture mediums for 24～48 h. According to the different colonies from the solid culture mediums, they were isolated and purified. G~+ bacteria and G~- bacteria were firstly confirmed under the microscopic and then the identified tests were carried out by ATB, automated bacterial identification and antibiotics susceptibility test system. (3) Each identified strains were randomly selected and injected intra-peritoneally to white mouse to examine their pathogenicity. (4) The antibiotic sensitivities of the pathogenic strains were tested with ATB. And (5) pathogenic strains were identified by PCR.RESULTS: (1) From the sows with endometritis, 44 strains of bacteria were identified in uterine cervix and the main strains were escherichia coli (27%), pseudomonas aeruginosa (16%), pseudomonas putida (14%) and A. hydrophila(7%). 26 strains of bacteria were identified in uterine horn and the main strains were escherichia coli(31%), pseudomonas aeruginosa(12%), pseudomonas putida (15%) and A.hydrophila (15%). From the sows with non-endometritis, only 2 strains of bacteria were identified in uterine cervix, they were escherichia coli (27%) and pseudomonas aeruginosa (16%). No any strain of bacteria was isolated in uterine horn. (2) Only the trains of pseudomonas aeruginosa and A.hydrophila were pathogenic after all the isolated strains were challenged to white mouse. (3) Antibiotics susceptibility test of the isolated strains of pseudomonas aeruginosa showed that the most of the isolated strains (70%) had strong resistance to antibiotics of penicillin,erythromycin,leucomycin,amox/clav.ac,oxacillin,cotrimoxazole, streptomy-cin,flumequine,kanamycin,oxolinic acid,gentamicin,fusidic acid,rifampicin, a few of the isolated strains (10%-20%) had strong resistance to pristinamycin, cafalotin, cefoperazone, apramycin, doxycycline.Antibiotics susceptibility test of the isolated strains of A. hydrophila showed that the most of the isolated strains (70%) had strong resistance to antibiotics of penicillin, erythromycin,amoxicillin, leucomycin, amox/clav . ac ,pristinamycin,oxacillin,tylosin,cft/cephalo.1G,cotrimoxazole,streptomycin, sulfamethi-zole,kanamycin,oxolinic acid,enrofloxacin, a few of the isolated strains (10%-20%) had strong resistance to colistin, cefoperazone, apramycin, furadantin, rifadin, doxycycline. (4) The main lesions of histopathology were the vacuolization of epithelial cell, the falling off mucous epithelium, necrosis in lamina propria and inflammatory cell infiltration.CONCLUSION: (1) High elimination rates of reproductive sows are related to sows' endometritis. (2) Environmental nature bacteria were conditional pathogenic bacteria of sows' endotremitis. Species and distribution of conditional pathogenic bacteria are various in different pig farms. (3) Antibiotics susceptibility of main conditional pathogenic bacteria of sows' endometritis are not the same and not consistency. But strong resistance to antibiotics, especially to the antibiotics major used in clinical practice are obvious in different pig farms. (4) The pathological change of sows with endometritis conformed to the pathological changes of inflammation process caused by bacterial infection completely.