Tissue Culture Study Of Three Carya Plants

The materials of this thesis are leaves and stems with buds of American pecan, Zhejiang pecan and Hunan pecan seedling and trees. The paper comprehensively and systemically studies on optimum culture medium, explants choosing, stem culturing, leaf culturing, browning of and so on. Primary results go as follow:1.It is supposed to be that the material of American pecan explanting cultivating is juvenile leaves, optimum culturing medium DKW, minimum hormone complex 2,4-D1.0mg/L+IBA2.0mg/L+6-BA0.02mg/L.2.In the browning proof experiment of American pecan explanting cultivating, the first step is normal sterilization by marinating the materials for 20 to 30 min with Na2S2O3 solution in 2%, and then dipping in the Na2S2O3 solution at 2% with autoclaving, which could lead to the best affect by taking out the materials before inoculation. The minimum browning rate appears by dipping the materials for 5 min with PVP solution at 300mg/L.3.In the browning restraining experiment of American pecan leaf explanting cultivating, it would be the best browning proof by putting the inoculated materials into the refrigeratory at 4℃for7days. Meanwhile, the minimum browning rate appears when the cultivating medium is added with Na2S2O3 solution above 5.0mg/L or PVP solution at 300mg/L.4.Pollution can be effectively controlled when American pecan leaves are pretreated with penicillin and the cultivating medium is added with Carbendazol solution at 4g/L.5.While cultivating stem of Zhejiang pecan, optimum culture minimum with hormone complex of bud growth differentiation is DKW+IBA2.0mg/L+KT8.0mg/L, and the one of leaf culture is WPM+NAA2.0mg/L+6-BA1.0mg/L.The optimum cultivating medium with hormone complex of Hunan pecan leaf is DKW+ IBA0.5mg/L+KT2.0mg/L, while the one of Hunan pecan leaf is MS+NAA1.5mg/L +6-BA3.0mg/L. The optimum saccharum concentration in cultivating medium of both Zhejiang pecan and Hunan pecan is 30g/L. 6.During the browning proof experiment of Zhejiang pecan and Hunan pecan stem apex, it is showed that browning can be restrained by medium added with Na2S2O3 solution at 5.0mg/L.7. About browning proof of leaf tissue culture, optimum basic cultivation of both Zhejiang pecan and Hunan pecan is WPM, while optimum antioxidant is Na2S2O3 at 2.0mg/L, which could lead to the best affect by putting the inoculated materials into the refrigeratory at 4℃for 7 days and then into the incubation room at 25℃. The lowest browning rate of Zhejiang pecan appears when materials are dipped in the PVP above 200mg/L while the one of Hunan pecan happens when materials are dipped in the PVP above 300mg/L.8. About succesive transfer culture, the available culture medium of both Zhejiang pecan and Hunan pecan is DKW. Optimum hormone complex of Zhejiang pecan succesive transfer culture is IBA1.0mg/L+6-BA0.2mg/L, and the one of Zhejiang pecan succesive transfer culture is IB A at 2.0mg/L. The optimum pH of both Zhejiang pecan and Hunan pecan in succesive transfer culture is 5.