Studies On Semen Cryopreservation For Arbas White Cashmere Goat

The principle of semen cryopreservation technique is based on the use of liquid nitrogen, dry ice or other cold source, the semen after proper processing, a complete cessation of a sperm cell metabolism can be achieved. The original part of sperm motilities can be recovered by rising temperature, so as to achieve a long-term preservation purposes. Then thawing the cryopreserved spermatozoa for artificial insemination or in vitro fertilization. Frozen semen can greatly improve the efficiency of utilization of breeder, reduce the cost of raising male livestock and is no longer restrictive with geographical, and temporal factors. Obviously, it is an effective technical means not only on breed improvment of livestock, but also on breeding and protection of endangered species. In conclusion, semen cryopreservation is of great significance on human reproductive medicine, livestock breeding and protection of endangered wildlife.Sheep semen freezing preservation technique, began with the 1950's; The study in this area in our country began with the mid-1960s. As for Goat, the semen cryopreservation technology research began with the mid-1970s overseas. However, since goat sperm is sensitive to temperature changing, it is so easy to be damaged of goat sperm function during the freez-thaw process, leading to the high effect of goat frozen semen hardly to repeat, great differences between the results of frozen semen, a lower pregnancy rate after artificial insemination by frozen semen of goats than that of natural mating and fresh semen, so it severely restrict the the popularization and application of goat semen inanimal production.1. Through comparing with the different dilution methods, it was found that in the room temperature close to 4℃, there was no significant effect on sperm motility between one-step dilution method and two-step dilution method. While as the temperature was higher than 4℃, sperm motility from two step method was significantly higher than one step method.2. By comparing the removal seminal plasma with unremoval seminal plasma, sperm motility of removal group was significantly higher than unremoved group, but theintegrality rate of sperm plasma membrane of removal group was significantly lower than unremoval group, that was caused by centrifuging sperm damage.3. By staining and flow cytometry assay, it was found that among the different individuals treated by the same formulaandthe same approach, their sperm plasma membrane integrity were not significant different. Whereas the rate of in advance capacitation and acrosomal integrity percentage were significantly different among the different individuals.