Study On Embryo Rescue Technique Of Munake Grapes Germplasm Innovation

Using seedless grape as material,4 crosses were carried out and the development of embryo was observed, after the embryo rescue of the seed from the crosses and self-pollinated. The self-pollination of white Munake grape, The self-pollination of white Munake grape, White MunakexMelissa, Red MunakexCrimson Seedless vestigial ovule were used as testmaterials in this experiment, achieved a theoretical basis of embryo rescue. The main factors for embryo rescue of Munake Grapes and germplasm innovation were studied. The main results are in the following:1. First established the culture system of embryo rescue technique on Munake grapes. The results showed that the best inoculation period of white Munake grape vestigal ovules was 40 days after flower drop stage. At the stage of ovule culture, the results indicated that Nitsch medium supplemtneted IBA 2.0 mg/L, GA3 0.5 mg/L,6-BA 0.5 mg/L, the percentage of ovules developed were the highest. Light training 60 days later, At the stage of embryo germination, Nitsch +6-BA 1.0mg/L+GA3 0.2mg/L+IBA 1.0mg/L medium, were mostsuitable for embryo development. At the stage of plantlet regeneration, the results indicated that the optimal medium of converting anormal plantlets was 1/2MS+IBA 0.1mg/L, the percentage of seedling survival were the highest.2. The observation of the development of the embryo showed that, the significant difference were found among the vestigal ovule in different DAF.Losing weight is fertile type ovule, first with the development of the ovule, present the increasing trend in 35 to 45 days, then reached a high began to decline. The heavier ovules weight training, when ovule vitro ovule, the higher the embryo development rate is high also, this germination level to embryo rescue is very meaningful.3. The study on the embryo rescue factor of infection showed that the best period for take out the embryo was depended on the ripe time of the parent female, early-ripen cultivar should be take out earlier then mid and later ripen cultivars, most of them were took out 40 days after flower drop. For the development of ovule, the best medium was Nitsch, while for the embryo germination, it were also Nitsch. Into seedling cultivation stage, the best medium was 1/2MS. After culturing the embryo on the development medium, Crosscut processing is suitable for embryo germination ovules processing mode. Adding proper of casein acids hydrolysate activated carbon and have certain promote ovules development role.4.125 seedlings were obtained by embryo rescue from four combinations among The self-pollination of white Munake grape, The self-pollination of red Munake grape, White MunakexMelissa, Red Munake×Crimson Seedless. According to the rescued seedlings transplanting system from the former researches,98 seedlings were transplanted, the percentage of seedlings survival was 89.7% on average.5. Using RAPD markers to chose 45 embryo rescue seedlings,21 seedless embryo rescue seedlings were chose. To provide a new seedless breeding germplasm of Munake grapes,and the system of marker-assisted breeding for seedless of Munake grapes was established.