| Shengji Powder collected in China Veterinary Pharmacopoeia 2005 edition are composed of sanguis draconis 30 g, halloysitum rubrum 30 g, olibanum 30 g, os draconis 30 g, borneolum synthcticum 10 g, myrrh 30 g and catechu 30 g. It has the efficacy of regenerating tissue to heal wound and is used to cure suppurative wound in traditional Chinese veterinary surgery. We are commissioned by the office of Ministry of Agriculture pharmacopoeia to evaluate the pharmacology and toxicology of Shengji Powder, which offers experimental data for the deep study and the application in clinical.Skin irritancy test In the experiment, we daubed Shengji Powder on the back of Guinea pig to observe the irritant action through erythema and edema by single test or continuous test. The results showed that there was no erythema and edema in intact skin and broken skin by single test, which score was zero indicating no irritation. Erythema appeared at 1 h and 24 h post-medication with no edema in continuous test, which score was between 0.6 and 1.0 indicating mild irritation. The irritant action disappeared at 48 h and 72 h post-medication.Long-term toxicity test The long-term toxicity was observed through being daubed on the back of wistar rats for continuous 4 w (6 h/d) with items of body weight, organ coefficient, hematology indicator, biochemical indicator and histological changes. The results showed that the rats had good condition, growth and development was normal, and the items of organ coefficient, hematology indicator, biochemical indicator, histological changes were all in normal conditions. It proved Shengji Power had no long-term toxicity and higher safety in clinical application.Suppurative wound healing activity test Suppurative wound was made of full-thickness skin cutting which was infected with staphylococcus aureus in the back of rat. Shengji Powder was made into ointment and daubed in suppurative wound for 5 days and 10 days. The results showed Shengji Powder Ointment could promote wound healing. Granulation tissue was detected to prove that Shengji Powder accelerated granulation tissue maturation, promoted blood capillary and fibroblast proliferations, and increased the secretion of Coll and Colâ…¢.Bacteriostasis test in vitro The active ingredient of Shengji Powder extracted by 70% ethanol was dissolved in DMSO to make 396.25 mg/ml stock solution which was 2-fold diluted to assay the MBC of Shengji Powder for staphylococcus aureus. The results indicated it could restrain and kill staphylococcus aureus with MBC of 6.20 mg/ml.Anti-inflammation test Mouse auricle inflammation caused by xylene and rat voix pedis inflammation caused by albumen were used to examine the anti-inflammation effect of Shengji Powder.70% ethanol was used to dissolve active ingredient to make 200 mg/ml stock solution. Pre-medication of 200 mg/ml stock solution had marked anti-inflammation effect, which significantly inhibited inflammatory edema caused by xylene with inhibition ratio of 50.31% and also markedly restrained inflammatory edema caused by albumen at 0.5,1,2,4 and 6 h.Activating blood circulation to dissipate blood stasis test Blood stasis model was made through mouse auricle microcirculation disorder induced by adrenalin.100 mg/ml Shengji Powder extract stretched the arteriole and venule caliber, accelerated micrangium blood velocity at 5 min after being dripped on mouse auricle and this effect lasted for 20 min above. The results indicated that Shengji Powder increased the micrangium caliber shrinked by adrenalin, accelerated micrangium blood velocity, and improved local microcirculation.Analgesia test In mouse writhing test, pre-medication of 200 mg/ml Shengji Powder extract markedly decreased the writhing response frequency causead by acetic acid with inhibition ratio of 53.73%. It was also proved that Shengji Powder extract increased pain threshold of 5.8 s, but duration was short in hot board responses test. The results showed that Shengji Powder had marked analgesic effect to pain caused by inflammation, while it had a little effect in mouse hot board responses participating with high-grade nerve centre.In summary, Shengij Powder showed a little irritative effect on skin, which also had no long term toxicity and higher safety in clinical application. Shengji Powder accelerated granulation tissue maturation, advanced blood capillary and fibroblast proliferations, increased the secretion of Coll and ColIII to promote wound healing. It also improved local microcirculation, restrained and killed bacteria in wound, eliminated excessive inflammatory reaction, and relieved pain to synthetically develop the effect of promoting wound healing. |
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