The Physiological And Biochemical Study Of Elymus Seeds During Ultra-dry Storage

This paper was focused on providing the ultra-dry storage theory and technical guidance of Gramineae seed. Elymus seed was chosen as trial materials.The silica gel was used to dry the seed, and the seed treatments in different moisture content (1.256%,2.579%,3.825%,4.474%,4.968%,6.097%,7.689%)were got. Then the seed was storied in aluminum foil bags at -4℃,4℃,20℃(Room temperature),45℃respectively for 1 year. Germination percentage, relatively conductivity, the anti-oxidation enzyme system, MDA content were tested during storage period. On the basis of analyzing ultra-dry seed Physiobiochemical changes, the relationship between the most suitable temperature for seed preservation and optimum MC was discussed. The results were as fallows:After stored the Elymus seeds in short term(1 year),-4℃ultra-dry conditions, there is no notable differences in seeds except the1.256% moisture content seeds have a relatively higher electrical conductivity and lower activity of POD. It shows that except 1.256% moisture content seeds, other seeds are stored well in -4℃. Compared with seeds(with 9.030 % moisture content)which have a lower germination percentage, GSH content but higher MDA content, there are no notable differences in all index of other seeds moisture content, This indicates that except the seeds which have a higher moisture content, other seeds can be stored well in -4℃. When the Elymus seeds is stored in 20℃, the seeds which have moisture content between 2.579 % and 4.474 % can keep a higher germination percentage, SOD activity, and lower MDA content. This indicates that the best moisture content in 20℃for Elymus seeds is between 2.279 % and 4.474 % when the Elymus seeds is stored in 45℃within 1 year storage period, the seeds with a moisture content of 2.579% keep a higher germination percentage, POD activity and lower MDA content. It shows that the best moisture content for storing Elymus seeds in 45℃is 2.579%.Through storage temperature,time, moisture content three factor analysis,it indicated that the best storage temperature is -4℃,the best storage is 6 or 9 month,the best moisture content is 3.825%,4.474%.Besides these results, the test also shows that neither higher(9.030%)nor lower(1.256%)of moisture content is not good for the preservation of Elymus seeds.In appropriate moisture content, Elymus seeds have good storage conditions. It is feasible to utilize ultra-dry storage theory to store Elymus seeds.