| This paper mainly studied the feeding responses to C. thermophile fibre enzyme in sheep and the degradation of coarse fodder in vivo and in vitro. By four tests, the article proved that the enzyme has action in sheep. The results showed:1. The effect of the cellulase complex on degradation of fibre in the corn stovers at 20℃, 40℃and 60℃were studied. The results showed, (1) The different temperature had significantly effected on the degradation of fibre(P <0.05), which was better at 60℃than at 20℃or 40℃; (2) The effect on the degradation of fibre was different with the dosage of the enzymes(P <0.05), which was better for the level of 0.9% than the level of 0.3% and 0.6%.2. Four Small Tail Han-sheep with average weight of 45kg fitted with permanent ruminal fistulae were used to study the stability of C. thermophile cellulase. C. thermophile cellulase was treated in vitro by ruminal liquid of sheep. The results indicated that the cellulase was stabler.3. Four Small Tail Han-sheep with average weight of 45kg fitted with permanent ruminal fistulae were used to study the effects of C. thermophile cellulase on rumen fermentation and the degradation of coarse fodder by vivo and nylon bag technique. The experiment was performed in 4×4 Latin square design. The results indicated that, (1) the VFA concentration was significantly increased(P <0.05), pH values were reduced(P <0.05), which was better for the level of 0.6%; but the ruminal ammonia-N concentration had no effect; (2) the enzyme significantly increased the degradation of fibre in the corn stover and wheat stalk(P <0.05); (3) the different types of coarse fodder have different degradations in the rumen of sheep, which was better for the corn stover than the wheat stalk in the same level of the enzyme.4. Eighty Small Tail Han-sheep were used to study the effect of the production performance of sheep by vivo. The results showed, (1) the total... |
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