Study On Establishment Of In Vitro Regeneration System Of Ornamental Pumpkin

In this paper, effects of plant growth substances, varieties and explants on callus induction, adventitious bud and root induction were compared using ornamental pumpkin, and an efficient in vitro plant regeneration system in ornamental pumpkin was established.1. Obtainment of sterile seedlings of ornamental pumpkinThe ornamental pumpkin seeds were selected as primal material and cultured on 1/2MS medium to obtain sterile seedlings. The results showed that pretreatment that seeds were dipped in 55℃water for 10 minutes and continuous dipped for 8 hours was of benefit to increase the rate of germination by 18 percent.2. Establishment of in vitro plant regeneration system in ornamental pumpkinThe effects of plant growth substances and explants on the organogenesis of ornamental pumpkin were investigated by using the orthogonal test. The results showed that the optimal medium for callus induction and callus growth was similar, MS+5.0 mg/L+0.5 NAA mg/L ,BA was the most important factor for callus induction, then NAA and explants. The cotyledon was the best explants for callus induction.The rate of adventitious root induction was influenced by many factors, among which NAA was the key factor. The suitable medium for adventitious root induction was MS+1.0NAA mg/L.Based on the above-mentioned results, a simple protocol for plant regeneration of ornamental pumpkin was developed: cotyledon explants were cultured on the medium of MS+5.0 mg/L+0.5NAA mg/L, and then the adventitious buds were cut and transferred to the medium of MS + 1.0 mg/ NAA. Some adventitious roots were produced and perfect plantlets were formed.This experiment is to obtain adventitious buds from explants without crypto gemmy or axillaries bud, and establish the in vitro plant regeneration system ornamental pumpkin.