A Study On The Callus Induction And Contents Of Bioactive Components Of Salvia Miltiorrhiza Bunge

Taking Salvia miltiorrhiza Bunge as an object, factors influencing callus induction and contents of bioactive constituents were studied, such as exogenous hormones, type of explants, pH of the medium, nutrients, etc. The results are as follows.1. To study the effects of exogenous hormones on the callus induction, S. miltiorrhiza aseptic stems and leaves were used as explants cultured in solid MS medium supplemented with 2,4-D, NAA, 6-BA or combinations of plant growth regulators with different concentrations. The results showed that all the three regulators could induce callus under certain concentration ranges. Better results could be obtained by combining three regulators, the optimum combination was Ms+2,4-D2.0mg/L+6-BA1.0mg/L. Higher concentration of 2,4-D (over 4mg/L) restrain callus growth.2. To study the effects of the type of explant on the callus induction, aseptic roots, stems and leaves were used cultured in solide MS medium. The results showed that the callus of leaves and stems could be induced with higher rate, better and quicker growth. The callus of roots however, were induced with lower rate and slower growth. The leaves were optimal explants for callus induction. The contents of salvianotic acid B and tanshinone IIA in the stem callus were higher, but cryptotanshinone was lower. The content of salvianolic acid B in the root callus was the lowest.3. To study the effect of pH on the callus induction, aseptic leaves were used as explants cultured in solid MS medium.The results showed that callus could be induced in the medium with different pH. The optimal pH was 5.8, the callus of leaves with high induction rate and better growth, but lower content of salvianotic acid. With the pH growing, The callus became harder, the colour turned from light-green to green when the pH increased. Both PAL bioactivity and content of salvianolic acid B were higher in the medium with pH 6.2. PAL could reflect the content of salvianolic acid B in a certain extent. The optimal pH could promote callus growth, but restrain the accumulation of secondary metabolities, and reduce the production of salvianotic acid B.4. To study the effects of nutrients on the callus induction, aseptic leaves were used as explants cultured in solid MS medium. The results showed that all the nitrents tested had...