Studies On Microsatellite Polymorphism Of OLA And Their Relationship With Growth Traits

The genetic variation of 6 microsatellites DNA in 218 sheep including Small-tailed Han sheep,Poll Dorset sheep and Texel sheep were investigated. The association between genetic markers and Growth traits were analyzed with least square analysis, the results are below:1. The number of alleles observed at there loci varied between four and sixteen. All microsatellite loci are highly polymorphic, with the PIC ranging from 0.3599 to 0.8315. Among these 6 microsatellite loci, DRB1, DRB2, DYMS and OHCC1 are high polymorphic loci, MB026 and BFMS are moderate polymorphic loci.2. The microsatellite variation statistics indicate that DRB1 has the largest total genetic heterozygosity (0.8417). While BFMS has the least total genetic heterozygosity(0.3576), The level of genetic variation within population of Small-tailed Han sheep was the highest, Dorset sheep and Texel sheep have no significant difference.3. The Hardy-weinberg equilibrium test showed that all the 6 loci for genetic diversity analysis within the three populations are not in equilibrium states.4. Effects of 6 microsatellite loci on growth traits were analyzed by PRCO GLM of SAS. Results show that the six loci have no significant association with the body weight. The effect of each factor on body weight of Dorset sheep is not significant either(P>0.05), Sex has a significant effect on weaning weight(P<0.05); The effects of age and breed are the major ones for body measurements traits, DYMS and BFMS have significant association with the cannon circumferend also.