Analysis On Genetic Diversity Using 20 Microsatellite Markers On Sheep Population In North Xinjiang

To reveal the genetic diversity and systemic relationship of 294 main sheep breeds in North Xingiiang,the genetic polymorphisms of 20 microsatellites in 9 sheep breeds in North Xinjiang were studied by means of PCR,polyacrylamide gel electrophoresis and silver staining. It can be offer the basis of protection and use of sheep breeds according the genetic variation degree of between and within population.The 9 local sheep populations in Nouthern Xinjiang were analyzed with the 20 microsatellite markers. The results showed that the BM1824 and ILSTS002 were low polymorphic markers, the other were high polymorphic.The total of 171 alleles were detected, the mean allele number was 9.5. Heterozygosity was 0.783, Polymorphism information content was 0.754,genetic heterozygosity was 0.818 and the effect allele number was 4.94.We analysis the gene flow between population and Nm was7.102,FST was 0.0434. The results showed that the gene flow was high of 9 sheep populations in Nouthern Xinjiang.We must protection the groups and the variety. According to Ds we know that Merino,Fine-wool sheep and other 7 sheep populations were relatively larg.We also know that Bashibai sheep and first filial generation(F1),second filial generation(F2),third filial generation(F3) were relatively larg. The genetic distances of Kazak,Aletai,Bashibai and Yemule were relatively small.The phylogenefc relationship between diferent sheep breeds was in accordance with their resourse, breeding history,diferentiation and localities.