Microsatellite Polymorphism For Xiaoweihan Sheep With Three Breeds Of Mutton Sheep Abroad And Two Their Hybrid Offspring

Abstract: The genetic diversity for Borderdale , Poll Dorset, xiaoweihan sheep and menggu sheep wasstudied using 12 microsatellite DNA markers, in which 4 of the markers are on the 18 chromosome ofsheep which was concerned as having association with Callipyge gene .The genetic heterozygosity,polymorphism information content(PIC), private alleles frequency , dominate alleles frequency , andNei Standard Genetic Distance were determined to evaluate the genetic variability within breeds andgenetic relationship among breeds . The results were as follows:The genetic diversity for Borderdale . Poll Dorset , xiaoweihan sheep and meng-gu sheep was studiedusing 12 microsatellite DNA markers ,in which 8 of them have abundant polymorphism informationcontent(PlC) and 108 alleles are detected .The polymorphism information content(PIC) of each site was abundant in 4 populations , the minimumPIC was 0.6 . The PIC for Borderdale , Poll Dorset , xiaoweihan sheep and menggu sheep was 0.823 ,0.838 , 0.825 , 0.835 respectively.The maximal Nei Standard Genetic Distance among them was between Borderdale and Xiaoweihansheep ,the follows in order were respectively Poll Dorset and Xiaoweihan sheep, Borderdale andmenggu sheep, Poll Dorset and menggu sheep, xiaoweihan sheep and menggu sheep, Poll Dorset andBorderdale .The site of CSSml 18 has significant (p<0. 001)association with body weight and body height of PollDorset, in which the genotypes for 146 and 146130 in body weight at three months were significantly(p<0. 001) higher than that of genotype 130; the genotypes 146 and 135 in body height at three monthwere significantly(p<0. 001) higher than that of genotype 130.