Studies On Molecular Cytogenetic Detection And Fertility Restoration Performance Of Male Sterile Line Of 1BL/1RS Wheat With Ae.kotschyi Cytoplasm

In this paper, using nine male sterile lines ms(Ae.kotschyi)-5-1,ms(Ae.kotschyi)-80(6),ms(Ae.kotschyi)-Yan Shi No.9,ms(Ae.kotschyi)-83(37)65,ms(Ae.kotschyi)-8222,ms(Ae.kotschyi)-77 ( 2 ),ms(Ae.kotschyi)-7-21,ms(Ae.kotschyi)-G-136,ms(Ae.kotschyi)-Xi'an No.9 as the materials. Identify alien chromosome and estimate the length fragment. In addition, choose six wheat varieties or lines as male parents to hybridize with the male sterile lines to study the test crossing and fertility analysis and their relationship with the length of translocation fragment. The results are as follows.1. The method of combined application of hydroxyurea(HU) and trifluralin was selected for synchronization of metaphase cells in root tips of wheat. The result was shown that metaphase index could reach to 70%-80%. It is quick and convenient to make chromosome flaking.2. Using the method of counting satellite chromosome,c-banding and genomic in situ hybridization to identify alien chromosome and estimate the length of the fragment. The result shows that the nine male sterile lines are all 1BL/1RS wheat male sterile lines and the translocation fragment of 5-1,8222 and 7-21 are shorter than others.3. The performance of restoring degree differed in different restorer-sterile lines hybridized with male sterile lines and it is significance of difference among male sterile lines with different nucleus. To analyze in the condition of the same male sterile line, it is significance of difference among male parents. It shows that the fertility restoration was related to the distribution difference of the series components of the fertility gene in individual nucleus of male sterile lines and their restorer lines in different wheat varieties or lines.4. There have no invariable relationship between the frequency of haploid of 1BL/1RS wheat male sterile lines with the cytoplasm of Ae.kotschyi and the hybrids deriving from the male sterile lines and six male parents. A further compare analyze shows that there are there relationships. They are negative correlation, coherence of frequency of haploid and positive...