Effects Of Medium Components On Fermentation Products And Plant Test Results Of Trichoderma Harzianum H-13

The over-used of chemical fertilizer and the pesticide had caused inadequate organic matter,hardening of soil,organism's structure worsened,cultivation environment destroyed,leading to cultivation,the organic phosphorus and chlorine of agricultural product gone beyond standard.In recent years,according to "the sustainable agriculture ",which was brought forward by the international,our country's bio-fertilizer research and exploitation developed fastly,brought up the plan of "eco-agriculture ", microorganism-fertilizer research and development was advocated,which will improvement soil structure and controll residual pesticide beyond standard.Trichoderma harzianum is the most widely used fungi in Trichoderma,it can adjust crop growth,its fermentation product can increase crop-related enzyme's activity and resist aging effects(such as nitratase's activity increased,MDA's content decreased,etc.).it also can promote plant uptake nitrogen,phosphorus and potassium;Meanwhile it can inhibit proliferation of pathogenic fungi,optimize the soil microbial environment,reduce the incidence of pests and diseases with the rhizosphere microbial interactions,so it can promote plant growth.This research used H-13,which is selected after N⁺ iron implantation Trichoderma harzianum,for the fermentation bacteria.Measure biomasses of bourgeon and growth with a small pot field experiments.Research H-13's culture conditionsfrom temperature,pH, incubation time,substrate media,the source of carbon and nitrogen,the ratio of carbon and nitrogen by orthogonality experiment.Study the liquid fermentation conditions from the incubation time,carbon and nitrogen sources and trace element.The results showed that the optimum conditions of sporulation and mycelial growth are pH7,26℃,7 days and pH7,28℃,8days,respectively.The optimum fermentation time is 9days.the optimum ratio of carbon and nitrogen is 20:1;organonitrogen is more suitable for H-13 growth than inorganic nitrogen,the best nitrogen of medium for mycelial growth and sporulation is hemoglobin;Nitrate inhibit generation of fermentation products. In Carbon,monosaccharide is good for mycelial growth,glucose/fructose(1:2)as the best; Disaccharide is good for sporulation,cellobiose/maltose(2:1) as the best.the output of GA is within 0.2-0.3g/L in different carbon,maltose is the best for form of GA,the output can get 0.308g/L;according to the dry weight of mycelium,cellobiose is benefit for mycelial growth during the stage of fermentation,the kind and quantity of trace Elements can impact synthesis of fermentation product,MgSO₄.7H₂O is most serious.The optimum conditions for the form of gibberellin are FeSO₄100mg/L,MgSO₄.7H₂O1g/L, K2HPO₄0.4g/L,NaNO₃6g/L,CaCO30.2 g/L,but this conditions don't impact dry mycelium.Spraying diffirent concentration of fermentation product on cucumber leaf,measure nitrate reductase,MDA,chloroplast pigment,peroxidase,superoxide dismutase(SOD),the results show that when concentration as 2.4～4.8μg/L,nitrate reductase activity can get 4.807μg/g.h,MDA content is only 4.155μg,The chlorophyll content is 1.746mg/g. Superoxide dismutase activity get 341.95μgF.W/min,Peroxidase activity get 50μg /(g.min).Spray diffirent dilution multiples of fermentation on different stage of cucumber,the results show that the best conditions for increasing cucumber biomass are the 100 multiple as the best concentraton and trophophase as the best spraying stage,concentration of spraying the best original broth diluted 100 times.When spray fermentation in reproductive stage,it will inhibit growth of cucumber,and increase the death rate of fruit; Seed germination rate is 97.33%,germination potential is 98.8%,the long of plant is 5.48cm,the long of rootis 5.12cm,dry weight of seedling is 8.87mg.After transplantation about 65days,the heightof plant up to 79.35cm and begin fruiting.it is earlier one week than the control group,solid peak for four weeks,it prolong two weeks than the control group.but fermentation did not affect single plant fruit.The major factor of increasing is the sex ratio increased,death rate of fruit decreased and fruiting production increased, the extent of incresing is as high as 50.8%.To sum up,H-13 inoculate in the culture with the content of 0.1%hemoglobin and cellobiose / maltose(2:1),after culture 7d at pH7,26℃,it was switched into another liquid culture,which 0.1%hemoglobin,sucrose / maltose(2:1),FeSO₄ 100mg/L, MgSO₄.7H₂O 1g/L,K₂HPO₄ 0.4g/L,NaNO₃ 6g/L and CaCO₃ 0.2 g/L in it,then spray the cucumber with the 100 multiples of fermentation dilution at trophophase.With the combination descried above,it is logic for the results of-differents concentrations and increased 50.8%per mu.