Study On Vitro Culture And Sugar-free Micropropagation Of Gardenia Jasminoides Ellis

Gardenia jasminoides Ellis is one of the main economic tree species in China. With a wide range of uses and a high value, it is a very good tree species which is gathering medicinal, ornamental and chemical value in itself. The elite plantletss of G.. jasminoides can't satisfy the demand for market now. Adapting in vitro propagate technique to magnify the plantlets will be a good way to settle this question. Some fragmentary researches have been worked out at the vitro propagate technique for the breeding of the G.. jasminoides ,but the research methods depended on experience, the materials was not an elite tree, and the sugar-free micropropagation haven't been attempted to.In this experiment well-bred seeds chosed from 3 elite families were used as the mateiials. The orthogonal design and the sugar-free micropropagation created by Fujian Agriculture and Forestry University were adopted to launch the vitro rapid propagation of G.. jasminoides in the every technique sector, including the bourgeon, subculturing and root induction of asepsis plantlets, root induction by sugar-free micropropagation as well as transplanting and surviving of rooted plantletss. In the every sector, better way and medium were dicussed and the key techniques were analyzed, which could provide a solid theoretical foundation and a basis for large-scale industrial production for the vitro rapid propagation of G.. jasminoidesThe main results were described as follows:1．The better methods on asepsis bourgeon of G.. jasminoides were selected, which included in turns the best predisposal by soaking the seeds in 40～60℃water for 24h before propagation; the best sterilized method by soaking the seeds in 6% NaClO for 8～10min firstly and then 0．1% HgCl₂ for 6～8min;the best basic culture medium by MS or B5 to obtain ideal sprouting effects.2．By analyzing the status of the plantlets in the first culture medium, the best medium being selected of the first culture was MS+NAA1．0mg/L+BA1．0mg/L +KT1．0mg/L +IBA1．0mg/L. The variance analysis of the effect of first culture among the different strips of G. Elli showed that the NO.3 strip had the highest multiplication rate.3．After transferring the plantletss were transfered into the second subculture medium from the first medium, the plantletss growth status was analyzed and the result showed that the best medium of the second subculture was MS+NAA1．5mg/L+BA3．0mg/L+KT1．0mg/L. The medium containing high concentration of BA would lead to large calluses at the base of the plantlets stem, which would take an adverse impact on continuous multiplication of G. jasminoides Thus the concentration of BA should be reduced even without it in the following experiment to improve the multiplication rate.4．By optimizing the medium on the basis of the second subculture the best medium for the continual culture was selected to be MS+NAA0．8mg/L +KT0．8mg/L.5．In rooting induction the best medium for the rooting selected is MS+NAA0．5 mg/L+IBA1．0 mg/L.6．The results of the experiment of sugar-free micropropagation induced rooting showed that the best medium for the rooting rate of G.. jasminoides was soil+NAA1．0 mg/L+IBA1．0 mg/L and the root rate reached to 100%. Especially noted, the control of the light strength and CO2 concentration were very important throughout the culturing process.7．The survival rates of the plantlets in the greenhouse contained auto-sprinkling irrigation system were higher than in common greenhouse, at the same time the perlites were chosed as the ground-substance. The survival rate would reach to 96% when the balance of moisture on the surface of the leaves could be better controled.