The Research Of Plant Regeneration In Muscari

Muscari is one of the eximious bulbous ornamental plant which is native to Europe. Bulb scale, leaf segment and flower bud were cultured as the explants in order to investigate the shoot induction, differentiation, elongation, root development and somatic callus, embryos production in vitro by the way of organogenesis and somatic embryogenesis. The main results as follows:The bulb scales as explants were cultured on three different groups of media. We found that the bulb scale was suitable for the shoot induction and the regeneration plants could be got by the way of direct and in direct organogenesis. The most important factor was the plant growth regulator. Few and small shoots grew slowly on MS media containing low concentration BA. On MS media supplemented with 2.0 mg/L IBA and 2.0mg/L BA the production of shoots could achieve 80% and the most shoots emerged on MS media with 5.0 mg/L IBA and 5 .0 mg/L BA. Compact yellowish callus were induced on MS media with 0.1 mg/L or 0.2 mg/L 2,4-D and 1.0 mg/L BA, and then the shoots emerged from it by the way of indirect organogenesis. Adventitious roots developed after 30 days culture on rooting media and the seeding could be transferred to pot when the root was 2cm long after 3~5 days acclimatization. The bulb scale was separated into three parts from basal disc to top on average and marked as upper, middle and bottom part. The middle part was the most responsive for shoot induction, then the bottom part, and the top part was the last.Using the leaf segment and flower bud as explants, the shoots were produced on different media via direct organogenesis and indirect organogenesis. All the receptacle and pedicel could produce shoots on MS media supplemented with 0.1mg/L 2,4-D and 1.0 mg/L BA. The induction frequency of pedicel was 77.8% on MS media containing 5.0 mg/L IBA and 5.0 mg/L BA but the receptacle was 8.6%, whereas the induction frequency of receptacle could reach 100% on MS media with 3.0 mg/L IBA and 3.0 mg/L BA. Yellowish callus could be induced from the other parts of the plant, such as leaf segments, petal and ovary wall, and the favorable media were MS media with 0.1mg/L 2,4-D and 1.0 mg/L BA or 3.0 mg/L IBA and 3.0 mg/L BA.The somatic embryo could be induced from the callus when the leaf segment as explants was cultured on different media. On MS media containing 0.5 mg/L NAA and 1.0mg/L BA, the production of callus was 100%. The yellowish nodular callus and white friable callus were induced, and the white one is somatic callus. The white somatic embryo dropped from the callus after we used the some media for callus regeneration two or three times.A substainial groundwork for plantlet regeneration, rapid and mass propagation of muscari by the way of organogenesis and somatic embryogenesis was established in the study.