Research Of Direct Regeneration Of Floral Bud From Explants Of Muscari

Muscari Mill. is a perennial spring planting flower bulbs, which belongs to the Hyacinthaceae family, which is a perennial ornamental plant. Because it has 40 species blue flower, so it as monocots ideal material, so as to which provides the basis for revealing the formation mechanism of monocots blue flowers. But it often takes three years at least seedlings from seed to flowering, which limits the functional verification of plant genetic progress of the work to a certain extent, thus limiting the use of molecular biotechnology progress color breeding. The study investigatived the optimal tissue culture conditions for Muscari armeniacum to induce directly the regeneration of floral buds.In this study, Muscari armeniacum as a material, using the techniques of tissue culture to establish a comprehensive system of renewable technologies. Floral buds explants of M. armeniacum was used as materials. With 0.1 mg/L 2,4-D, the buds was treated with different concentrations of 6-BA(0,0.5,1,2,3,4 mg/L), GA3(0,0.5,1,2,3,4 mg/L)and Zeatin(0,0.05,0.1,0.5,1,2 mg/L), espectly. To select the optimal induction condition for bud differentiation. Using MS +6-BA(or ZT) 2 mg/L+ 0.1 mg/L 2,4-D effect of different explants on the optimal induction condition to flower bud were studied. The main results of the study are as follows:1. The optimal induction condition for bud differentiation was MS+2 mg/L 6-BA + 0.1 mg/L 2,4-D, the optimal induction explant is floral buds,with induction rate of 60.2%.2.With the same hormones conditions, pedicel, sliced petals, leaf and callus explants can only be induced callus, vegetative buds. But all of explants in GA3 and zeatin do not induce flower buds just callus and a small amount of vegetative buds. So the optimal induction explant is floral buds, optimal induction condition is MS+2 mg/L 6-BA + 0.1 mg/L 2,4-D.3. The effect of different concentrations of sucrose for induction of floral bud, the suitable sucrose concentration of inducing flower buds is 30 g/L.When the sucrose concentration is 60 g/L, the buds is induced the formation of callus. When the sucrose concentration is 90 g/L, a portion of the buds is browning death. When the sucrose concentration is 120 g/L, all buds is browning died.4. Through the different explants,different exogenous hormones to induce the occur of somatic embryo, the following conclusions : The most appropriate of explants for inducing somatic embryo is flower bud. The most appropriate of medium is MS + 0.1 mg / L 2,4-D + 1 mg / L 6-BA. The rate of differentiated somatic embryo is 71.1%. The time of occuring directly somatic embryos to plants is 90 d.5. To bud explants filter the Hyg concentration, when Hyg concentration is not less than 6 mg / L, explants is beginning browning. When the Hyg concentration is 10 mg / L, the rate of browning is 90.44%. Thereby determining Hyg concentration of floral buds of Muscari is 10 mg / L.6. The hardening of the regenerated plants were transplanted, transplant survival rate of 90%.