Study On The Sustained Release Preparation Of Chinese Traditional Compound Medicine "CNⅡ" And The Releasing Property In Vivo And Vitro

Chinese traditional medicine "CNⅡ" is a compound medicine which is aim directly at bacterial diarrhea ofanimal. "CNⅡ"can inhibit the growth and multiplication of E. coli, reduce the virulence, slow down the animals'enterokinesia, it has good curative effect to prevent and cure the bacterial diarrhea of animal. But "CNⅡ"bitterin taste, it's difficult to feed, it's not stability in GI, it's not easy to storage, it must be given medicine frequently.In order to improve the stability of the medicine, reduce the giving number of times, reach the blood drug levelstability, increase the effect of sick animal in clinic, the test makes the medicine, into sustained releasepreparation, it makes the medicine be contained inβ-CD. So it can improve the stability, cover up themedicine's bitter, reduce the giving number of times and keep the blood drug level stability. The experimentcontains the test of preparing the sustained release preparation, the test of assaying of sustained releasepreparation, the test of TLC, the test of pharmacodymaies, the test of releasing medicine in vitro, the test ofreleasing medicine in vivo.As the content of matrine index, in 50℃, 4h, CNⅡ:β-CD=1:3,can make out the best sustained releasepreparation; after the way of observation of microscope and the test of pharmacodynamics, the result indicate itwas succeed in clathration; the average content of sustained release preparation is 35. 564±0.12％; let the result ofsustained release preparation fit as zero order release, it can indicate as a functional relation equation:Y=6.5716X+9.8374, r=0.9932; let the result of sustained release preparation fit as the model of time square, theHuguchi equation: Y=25.903t^1/2-7.574,r=0.9882; using the trapezoidal rule to calculate, the Cmax and Tmaxwere true results, the metabolism parameter of the group of CNⅡwere: Tmax=3±0.17h, Cmax=26.79±6.12ug/ml, AUC_0～24=20.47±2.3ug/ml, MRT=3.48±0.09h; the group of sustained release preparationwere: Tmax=5±0.25 h, Cmax=22.454±5.21ug/ml, AUC_0～24=9.88±0.21ug/ml, MRT=5.50±0.12h;afier t analysis,the Cmax and AUC_0-24 ofβ-CD'CNⅡhave no significant deviation with CNⅡ(P＞0.05), but the Tmax was as1.67 than CNⅡ, they have very significant deviation in MRT (P＜0.01).The results indicate that the sustained release preparation of Chinese traditional compound medicine CNⅡhas the properly of sustained release compare with CNⅡ.