Studied On The Quality Of Semen Ziziphi Spinosae In Difference Areas Of Shaanxi Provence

Semen Ziziphi Spinosae(SZS),the dried ripe seed of Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou, is a commonly used traditional Chinese medicine(TCM )recorded in pharmacopoeia of the people's republic of China (from 1977 to 2000 Edition). It is sour to the taste, neutral in nature and distributed to the heart, liver and hall bladder channels. It has actions of replenishing the liver, causing tranquilization, arresting excessive perspiration and promoting the production of body fluid. The source complex and quality large difference in market made certain diffculty on clinic application of SZS. The quality of SZS in different areas of Shaanxi province(Qingjian in Shanbei areas, Chengcheng, Pucheng and Yongshou in Weibei area, Luonan in Shannan area) were compared with contents of active ingredients as the inspection indicator, Gansu (Jingchuan)as reference area, big SZS in Jiaxian as reference varietiy for studying the quality in the province. At the same time, the optimum extractive technology of total flavonoids and total saponins were studied. The results were as follows :Confirmed the optimum extractive technology of total flavonoids and total saponins,through the single factor (PH) and the five factors ( temperature of extraction, concentration of ethanol, time of extraction, stock ratio (mass : volume) and times of extraction) four levels orthogonal test, according to the charactors of flavonoids and saponins of SZS. The optimum extraction condition of total flavonoids was that temperature 75℃, adding 70% ethanol(PH6.5), stock ratio1:12, refluxing 3 times,1.5 hours pertime. The optimum extraction condition of total saponins was that temperature 85℃, adding 80% ethanol (PH6.5), stock ratio1:12, refluxing 3 times, 2 hours pertime.Analyzed the content of active ingredient in SZS, the result showed that fatty oil content reached 30%, witch contains plenty of unsaturated fatty acid, and the content of oleic acid and linoleic acid were higher than others. In degrease powder, total flavonoids content was 3.2～4.6 mg/g, jujuboside A 0.35 mg/g , jujuboside B 0.17～0.32 mg/g,and the total saponins content was higher than 40mg/g. There are large quantity and many types of amino acids in SZS, the glutamic acid content was higher than 47 mg/g, which has important function to human body, and the essential amino acids to human occupied 29% in the total. Compared the content of active ingredient in SZS and found the indexes existing difference in different producing areas and different years . The result showed that contents of fatty oil, total flavonoids and total saponins of SZS in Qingjian area were all higher than others, contents of jujuboside A and B distinguished lower than Chengcheng and Luonan. So, it considered that the qulity of SZS in Qingjian was better than others. In conclusion, every index stability and higher,the qulity of SZS in the north of shaanxi is better than others. There is no regularity in the midland area because of the verity of contents. The result has guidance significance to scientific utilization the resource of SZS in shaanxi province.The chemical constituent are compared between Ziziphus jujube Mill. var. spinosa and transitional type. It showed that the content of fatty oil is higher and jujuboside A content is lower than common SZS. The result can provide a certain reference frame for studying the relationship of evolvement.