Effect Of Cysteamine On The Antioxidation And Blood Fat Of Mice Fed High Fat

Feeds with high fat can reduce the rate of energy metabolize. Animals which fed high fat for a long time may induce hyperlipoidemia, obesity and so on. The aim of this study was to investigate the oxidative stress induced by high fat intake, and effect of cysteamine (CS) on body anti-oxidative ability and fat metabolism in animal.Male KM mice were fed with high fat feed (containing 4% or 8% of lard) added with 0, 24 or 48 mg/kgBW of CS for 3 month. It was shown that high fat intake decrease anti-oxidative ability of mice. However, when the feed was added with 24 or 48mg/kg of CS, superoxide dismutase (SOD) in serum, liver and gut was increased, and malonaldehyde (MDA) was decreased.To establish an in vitro mice oxidative stress model, oleic acid was added into primary liver cell to 0, 50, 150, 200 and 250μmol/L, ROS, glutathione, ATPase activity and MDA were determined. The result shows that 150μmol/L is the optimizational concentration. Then, primary were cultured with oleic acid (0,150,250μmol/L) and CS (0, 50, 100, 150, 200, 250, 300, 350, 400, 450 or 500μmol/L). It was shown that low CS could increase glutathione and ATPase activity, decrease MDA and ROS. When more oleic acid was added, more CS was needed to scavenge ROS. If more than 350μmol/L CS was added, the scavenging ability was decreased. In another study, primary liver cell was cultured with 250μmol/L oleic acid, and 0, 150 or 300μmol/L CS, DNA injury was studied using single cell gel electrophoresis, the results show that 150 and 300μmol/L of CS could significantly decrease occurrence percentage and length of DNA ladder。Results of in vitro and in vivo study reveal that CS could enhance anti-oxidative ability during fat induced oxidative stress, enhance free radical scavenging ability,decrease lipid peroxidation,increase fat metabolism and lipase activity.