Studies On Tissue Culture And Plant Regeneration Of Hypericum Forrestii N.Robson

The purpose of this study was mainly to probe an efficient way for tissue culture and rapid propagation of Hypericum forrestii N. Robson. The leaves, stems or shoot-tips of young branches were taken as explants. The main results were as follows:1. Primary cultureMS+6-BA2.0 mg/L+NAA0.1 mg/L was the best initiation medium in which the rate of initiation was 96.00%, and the shoots which came from stems grew very well.The best medium for induction of calluses from flower buds was MS + 2, 4-D 1.0 mg/L+TDZ0.03 mg/L+NAA 0.1 mg/L. The induction rate could reach up to 87.50%.2. Proliferation and differentiation cultureThe optimum proliferation medium for shoots from stems was MS+6-BA 2.0 mg/L+NAA0.1 mg/L, on which the shoots could multiply 4.25 times after 30 d.Highly proliferating calluses were obtained from the buds cultured on MS +6-BA3.0 mg/L+2, 4-D 0.1 mg/L+NAA 0.1 mg/L. The proliferation rates could be up to 90.00%.The proliferation couldn't be promoted by adding carrot juice, apple juice, tomato juice or banana juice in the medium. But all these juices could makethe shoots stronger.The best culture medium for shoot formation from calluses was MS+ 6-BA 2.0 mg/L + NAA 0.1 mg/L. The regeneration rate was 90.00%. 3. Rooting culture1/2MS+NAA 0.5 mg/L+IBA1.0 mg/L+AC3.0 mg/L was the best rooting medium, in which the rooting rate could be up to 100% and the average height of plantlets was over 5cm.4. TransplantingPlantlets were transferred to the medium consisting of moss. The survival rate was up to 93.33%.