Study On The Propagation Technology Of Agapetes Hillii

Agapetes hillii Brandis is an epiphytic evergreen shrub,which is mainly distributed in Xishuangbanna and Dehong,Yunnan,China.Agapetes hillii has high ornamental values for its beautiful flowers,various tuber shapes and edible fruits,and it also has high medicinal value for treating cancer.However,as a result of growing in inaccessible primitive forests close to the country border,although A.hillii has so high value of the potential market economy,it received little attention.At present,the plants of A.hillii were indiscriminately collected for business,which caused a great decrease in its populations.Thus,this research intends to study the breeding technology of A.hillii,and explore the method of its rapid propagation,so as to protect its natural resources,and to provide the experience of the resource development and utilization of it in the future.This study investigated the wild germplasm resources of A.hillii first,and then collected seeds and branches to conduct the experiments of seed germination,cutting propagation and tissue culture.The results of the study are summarized as follows:1.Through the investigation of the main distribution areas of A.hillii such as Jinghong City,Mengla County and Yingjiang Country in Yunnan,it was found that A.hillii only grows in thin forests or thickets of limestone mountains without disturbance,and the distribution altitude is from700 to 2100 meters.The associated plants of A.hillii mainly are those that also have an epiphytic habit,such as Bryophyte,Davalliaceae,Polypodiaceae,Neottopteris(Adiantaceae),Hoya(Asclepiadaceae),Gesneriaceae,Araceae,Cautleya(Zingiberaceae)and Orchidaceae and so on.Agapetes hillii and those plants grows on the tree trunks or stones together.2.The germination experiment of A.hillii seeds was carried out by using two factors: the origins and the storage time.The results showed that dormancy phenomenon did not occur to the seeds of A.hillii.The seeds that were just collected had the highest seeds germination rate,and the germination rate decreased gradually with time.The germination rate of the seeds from three origins were basically the same during storage,however,with the increase of storage time,the seeds from Mengla had higher germination rate than those from Jinghong and Yingjiang,which indicated that the seeds from Mengla had higher vigor and can be stored for a longer time.3.The cutting propagation experiment was carried out by using A.hillii branches under different conditions,such as ways of left lobe,ABT1 concentration,treatment time and substratum.The results showed that:(1)The rooting rate of branches with 3-5 leaves was the highest,which was 46.7%;(2)With the increase of ABT1 concentration,the rooting rate of the branches increased first and then decreased,and when the concentration was 100 mg/L,the rooting rate reached a maximum of up to 46.7%;(3)When we dipped the cutting into 100mg/L ABT1 for 10 s,10min and 1h,the rooting rates of cuttings were 26.7%,46.7%,and 40%,respectively,which indicated that the best treating time was 10min;(4)We used Vperlite : Vvermiculite : Vbark = 1:1:1 and sphagna as two cutting mediums of A.hillii branches,and the rooting rates were 30.0% and 46.7% respectively,which indicated that sphagna was more suitable for cutting rooting in A.hillii.4.Tissue culture experiment was conducted by using seeds of A.hillii as explants.(1)Explants disinfection: disinfect the seeds with 0.1% mercuric chloride by 10 min first,and then with 70% alcohol by 30 s.After,wash the seeds with sterile water for 4 times,and drain them on sterile filter paper.(2)Original culture: the sterilized seeds were cultured in MS medium.Similar to the previous results of seeds germination test,the germination rate of 0d(just collected seeds)was the highest,reaching 82.8%;seeds were stored in the refrigerator at the temperature of 4,the germination rate of 50 D was 53.3%,and the germination rate of 100 D was 10.0%.(3)Proliferation culture: through experimental comparison,the best medium for the proliferation culture was MS+6-BA 0.5mg/L+NAA 0.5mg/L,and callus rate was 82.8%.