Effects Of LDL And PCS On Cryopreservation And Contents Of Lipids In Goat Spermatozoa

For the lower pregnant rate of AI with goat frozen semen than that of natural mating, the goat frozen semen were still not used commercially. One of the reasons of lower pregnant rate might be the depletion of cholesterol from goat sperm during freezing leading to premature capacitation. Therefore, the paper studied on the effect of low density lipoprotein(LDL) and potassium cholesterol sulfate(PCS) to the extender on the cholestero, phospholipids contents of goat spermatozoa and their cryopreservation during Freezing Process. Firstly,LDL was used to instead of egg yolk in diluents to study on the protection of sperm from freeze. Additional, PCS was supplemented to LDL diluent and EY diluent to study on the ability of LDL+PCS and EY+PCS to maintain the cholesterol and phospholipids contents, motility, membrane and acrosome of sperm.1.When EY was replaced with LDL in goat semen frozen diluent, LDL could maintain the cholesterol content and the ratio of cholesterol and phospholipids. LDL could also increase the motility, the rates of no-effected embrane and acrosome of frozen goat sperm.So, LDL could be used to replace the EY in diluent, and the suitable dosage was 9g LDL in 100ml diluent.2.When PCS was added to the LDL diluent, PCS could increase the ratio of cholesterol and pgospholipid, motility and the rate of no-effected membrane of frozen goat sperm. PCS could be used as a composition in goat semen frozen diluent.3.In EY diluent, PCS could increase the ratio of cholesterol and phospholipid, motility and the rate of no-effected membrane of goat sperm. So, PCS could be used as a composition in gaot semen frozen diluent and suitable dosage of PCS was 0.10g/100ml diluent.