Study On The Association Of A-FABP And PRLR Gene Polymorphisms With Production Traits

PCR-SSCP and DNA sequencing were used in this paper to study the polymorphism of fatness candidate gene (A-FABP) and reproduction trait candidate gene (PRLR) in Beijng ducks and Cherry Valley populations.The associations of their polymorism with production traits were also studied. The aim of this study was to finel some genetic markers which associated with important economic traits; provide some imformation for breeding in native duck populations, establishment the database of molecular markers, maintenance and using breeds resources. The results were showed as follows:1. Relationship between genetic variation of A-FABP gene and production traits in meat-type ducks(1) SNPs of A-FABP gene were analysised, the results showed that there were no polymorphisms found in A1 locus.Six SNPs were found at intron 2 (EU306610:g.106A>T, 108T>C, 123T>C, 164C>T, 215C>A and 231T>C).(2) The analysis of population showed that there were some differences in alleles and genotypes among different populations. The frequency of A Allele was higher than B Allele, and there was significant difference in Z2 and Z4. But there were not significant differences in other populations.(3) Theχ2 test revealed that A2 locus was in Hardy-Weinberg equilibrium in Z4, cross and cherry valley populations (P<0.05), and was in Hardy-Weinberg disequilibrium in Z2 population (P>0.05). All populations were moderate polymorphic (0.250.05).2. Relationship between genetic variation of PRLR gene and laying traits in Beijing ducks (1) The study results of the diversity of PRLR gene in ducks showed that there was no mutation in P1 locus, and eight mutations were found in P2 locus. The results showed that there were seven genetypes.(2) The least square analysis results showed that in Z2 genetype F had higher laying number and laying rate. There were no significant differences in W2 population.