The Polymorphism Of PRLR Gene And Its Relationship With Fecundity Traits In Goats

Prolactin is a hormone produced and secreted by lactotropic cells of the Anterior pituitary gland.More than 300 kinds of functions had been reported.These functions was regulated by its receptor.Because of the important role of the PRLR,it was used as a strong candidate gene for reproductive trait in pigs.It was the one of the considerable economic trait of the fecundity.And the genetic factor was the one of the key factor to the fecundity trait.But the fecundity trait is low-heritability trait.It was not obtain major genetic progress to select the trait by conventional breeding method.So the trait was researched to search the gene to control the fecundity by DNA level.It was the fully significance to breeding the goat breed variety of the high fecundity.The polymorphisms of goat prolactin receptor gene(PRLR) was detected by PCR-SSCP method with different pairs of primers among goats 150 Tianfu goats,70 Boer Goats and 40 Chengdu Brown Goats(Acquisited by Sichuan Agriculture University and Huiyuan Animal husbandry Company).In this study,comparing the PRLR of sheep and human in GenBank,the primers were designed the tenth exon of different goat breeds amplified.There are two SNP sites in the exon 10 of PRLR gene.The Tianfu Goats and Boer Goats were midrange polymer-phism (0.25＜PIC＜0.5).The Chengdu Brown Goats were low polymorphism(PIC＜0.25).The result of x~2 fitness test indicated that Boer Goat was highly significanty biased from Hardy-Weinberg equilibrium(P＜0.01),but Tianfu Goats and Chengdu Brown Goats were all kept in Hardy-Weinberg equilibrium(P＞0.05).Among goats with littering records,least squares analysis showed that the three breeds Goats of AB and BB genotype yean number was less 0.221(P＜0.05) and 0.389(P＜0.05) than BB genotype.The Boer Goats genotype relation was AB＞AA＞BB and the Chengdu Brown Goats genotype relation was AB＞BB＞AA,but the both were not quiet discrepancy. The other genotype relation of three breed varietys was CC＞DD＞CD.The yean number of the Tianfu Goats and the Boer Goats CD and DD genotype were less than CC genotype and the both were quiet crepancy.The yean number of the Chengdu Brown Goats CD and DD genotype were less than CC genotype and the both were exceeding discrepancy.So the PRLR gene is possible primary gene to control fecundity traits of the Tianfu Goats,the Boer Goats and the Chengdu Brown Goats.But it will be confirmed by more research.