Effect Of Micro-Environment On The Growth Of Tuber Flowers In Vitro And The Study On The Technique Of Transplantion

In flowers garden, the tuber flower is favoriate by consumers because of its gigantic flowers, gorgeous colors and strong compatibility .They are not only used cutting flowers but also potted. Tissue culture is the main method which used to reproduce tuber flower. It can solve many questions that bulb breeding encounters, such as the lower breeding indexs, severe virus contaminated, but it is difficult to apply to factory-production, the main reason is the lower survival rate. Transplanting and domestication is the last part of the tissue culture process, and is the most critical aspect.So,deepen the study on the shape and physiological changes during transplanting and domestication is beneficial to the plantlets transplanting, improve the survival rate.Micropropagated hyacinth and lily were used to study their growth and physiological responses to sucrose concentration added in the MS medium,PPFD during rooting in vitro.And studied the influence of different exercising days, different bulb diameter, different matrix and different plantlets quality on the survival rate.The results were as following: 1.A regeneration system was initiated from Muscari botryoides"Album"and Asiatic hybrid lily"elite"Leaves and bulbs of Muscari botryoides"Album"were chosen as explants.Different type and concentration of hormone were studied in order to induce callus and adventitious bud. The results showed that the leaf was more appropriate explants for tissue culture of grape hyacinthus than the bulb. The optimum medium for inducing callus form leaves was MS+2,4-D 0.6 mg·L-1+6-BA 0.1 mg·L-1,the induction frequency may reach 100%;The combination of MS+2,4-D 3.0 mg·L-1+6-BA 0.3 mg·L-1 was the appropriate medium for bulbs forming callus with the induction rate 91.2%.The best medium for inducing adventitious buds from callus was MS+6-BA 2.0 mg·L-1+NAA 0.3mg·L-1,the germination rate was 97.4%;The medium of 1/2 MS+IBA 1.0 mg·L-1+1 g·L-1 AC was suitable for rooting with strong roots.Bulbs of Asiatic hybrid lily"elite"was chosen as explants. Different type and concentration of hormone were studied in order to induce adventitious bud. Then use leaves and bulbs of the new induced plantlets to establish regeneration system from Asiatic hybrid lily"elite". The adventitious shoots were induced from scales explants based on MS basal medium supplemented with 2.0 mg·L-16-BA and 0.2 mg·L-1NAA. The medium suitable for the leaves of the plantlets to differentiate adventitious bud was MS + 6- BA1. 0 mg·L-1+ NAA0. 2 mg·L-1;The medium suitable for the scales leaves of the plantlets to differentiate bud was MS+6-BA 2.0 mg·L-1+2,4-D 0.1mg·L-1, the induction frequency may reach 100%.MS+NAA 0.2 mg·L-1+0.2%AC was suitable for rooting.2.Effects of micro-environment factors in vitro on the growth of the bulb flowers and the survival rate after transplant during rooting stageNo-rooting grape hyacinth cuttings were cultured on solid Murashige- Skoog medium with 3% sucrose or without sucrose, lily cuttings were cultured with 5% sucrose or without sucrose, and in 3 photosynthetic photo flux densities(86,20,48mol·m-2·s-1) for 15,30 days respectivly.The results indicated that sucrose was beneficial to biomass formation of plantlets, higher PPFD was benefit to the growth of plantlets. TreatmentⅣwas the best for the growth potential of hyacinth and lily plantlets. With-sucrose raised the activity of roots and antioxidases, with-sucrose and higher PPFD all increased the content of Chl.. Light intensity has visible influence on the stomaal opening and closing. Along with the light intensity increased, the width of stoma also increased, but the density decreased. Sucrose has a certain degree influence on the stomaal opening and closing. On the same PPFD, the length and width with free-sucrose were all above that with-sucrose, the density was lower. The plantlets with sucrose,higher PPFD had the highest survival rate and better growth expression after transplant.3.Effect of different exercising days,different diamester bulb,matrix,different plantlets quality on the survival rateThe results showed: the bulb flower plantlets had a higher survival rate through 5 or 7 exercising days before transplant. Bigger the bulbs were, higher the survival rate; the plantlets transplanted on mixed matrix had the better growth potential, higher survival rate. Among those, the 1/1 Perlite/Peat was the most appropriate for the growth of hyacinth plantlets, the is 96%, the 1/3 Perlite/Peat was the most appropriate for the growth of lily plantlets, the survival rate is 98%;on the aspect of the plantlets quality, the survival rate of normal seedlings is higher than that of few roots seedling, seedling of no normal roots was the lowest.