| Starch properties are important factors to noodle quality. 99 spring wheat cultivars and lines from domestic and overseas were used to survey the waxy proteins subunits composition and waxy genes using 1D-SDS-PAGE eletrophoresis and molecular markers. RVA properties, agronomic traits, seed vigors were analysed. All for finding accurate, fast and simple method to screen good quality wheat resources and varieties. The major results were presented below:1. In total 99 wheat cultivars (lines), 19 cultivars (lines) with null Wx-B1 were presented in domestic and overseas wheats, and no Wx-A1 null cultivars. Xinkehan 9, Xinchun 6, Heyou 2, Wuchun 3, Longchun 15, Ningchun 29, Ningchun 31, Kenhong 14, Fengqiang 39 wheat materials can be further used in wheat breeding.2. Three molecular markers could be used to detect different waxy genes. A 1172bp fragment was detected for Wx-7A gene using dominant marker primer from the Wx-A1a normal wheat, a 593bp and 574bp fragment were detected using codominant marker primer from the Wx-A1 normal and Wx-A1 null wheat, respectively. A 425bp fragment was detected for Wx-4A gene using dominant marker primer from the Wx-B1a normal wheats, whereas this fragment was absent in the Wx-B1 null wheats.3. Differences between waxy protein and molecular markers were observed. PCR amplifidation using Wx-A1 dominant selectable marker primers was match with the results of waxy protein subunit electrophoresis in most of experimental materials, few materials were detected Wx-A1 protein, but not amplified corresponding specific DNA fragments.4. Significant variations in starch properties were observed. Coefficient variation in peak viscosity, hold through, total setback and final viscosity were 38.10%, 68.24%, 52.14% and 59.78%, respectively. The coefficient variation of peak time was the lowest in these parameters and it was only 9.74%. In seven pasting traits, significant correlation was detected among the six pasting traits except pasting temperature. Peak viscosity had high correlation with holding strength,breakdown, final viscosity, peak time and setback, their correlation coefficients were 0.957, 0.626, 0.959, 0.938 and 0.915, respectively. Holding strength also had high correlation with breakdown, final viscosity, peak time and setback, their correlation coefficients were 0.374, 0.992, 0.958 and 0.953, respectively.The difference for hold through and final viscosity and total setback between 19 cultivars with null Wx-B1 and the others with normal subunits was at 5% significant level, and peak time, peak viscosity, breakdown, paste temperature had not significant difference. significant variation was observed in starch properties in Chinese wheats and the range for peak viscosity, breakdown, final viscosity, total setback and hold through was 981～3990, 748～1744, 405～4294, 264～1836 and 141～2458, respectively.5. Analysis on seed vigor variances showed that dry weight, fresh seeds, germination rate, root length, bud length, germination index and vigor index had distinct differences among 16 various varieties. The vigor index of 99J-259 and D68-20 were the highest than the others.6. Wx genetic variations had no significant correlation to plant height, ear length, the effective number of tillers and panicles per plant, grain number per spike and 1000-grain weight. The heigher plant of waxy wheat was associated with agronomic characters of their hybrid parent, not with waxy gene mutations. The main agronomic characters variance analysis showed that spikelet number per spike had significant differences among the 18 Wx-B1 deletion wheat varieties. Panicles per hill and grain number per spike could be used in high yield wheat breeding.
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