Establishment Of Regeneration System For Acacia Fimbriata

Acacia fimbriata belongs to Mimosaceae family and Acacia genera.It has well developed root nodule,strong ability of nitrogen fixation,high biomass and multiple litter,which has very good protection and modified effect on soil.With the development of our country park business, the ornamental acacia which has ecological value and significant value will necessarily attract more people's developing and studying interest.In this paper,the establishment of aseptic system, proliferation culture,induction of root and acclimatization and transplants etc were researched,the key factors that affecting proliferation rate and root induction rate were analyzed,the rapid propagation technique system in vitro was establish,using the imported seeds from Australia whose species source number was 15472 as explants to establish,in order to offer theory foundation for factory breeding.With single factor,orthogonal design and analysis of variance to find out the optimum explant and best medium composition and the culture process for the culture of Acacia fimbriata.The main results were described as follows:1.The establishment of aseptic system of Acacia fimbriata.Treated with 1.84g/mL concentrated sulfuric acid for ten minutes,the seed's germination rate was up to 53%after ten days,83%after thirty days,and the germination effect was good.2.The infection rate of Acacia fimbriata seeds by using different sterilization time.Using concentrated sulfuric acid as first treatment,it could effectively kill the bacteria and fungus around the seed.The routine disinfection was soaking for ten second with 75%alcohol,then sterilizing in 0.1%mercuric for ten minutes,finally washing with sterile water for five times.The infected rate was only 6%.3.Proliferating culture of Acacia fimbriata.The method of orthogonal design was adopted to arrange experiment.Considering the influence of plant auxin on the proliferation culture of Acacia fimbriata,selected MS+1.0mg/LBA+1.0mg/L KT+0.4mg/L IBA as the first culture medium,the proliferation coefficient was 3.61.The Acacia fimbriata seedling grew worse in the second multiplication culture,the highest proliferation coefficient was 3.72 with the medium combination of MS+0.4mg/LBA+0.4mg/LKT+0.2mg/L IBA+AClg/L+20g/Lsucrose.During the third and multi-generation multiplication culture,taking the MS+0.4mg/LKT+0.4mg/LIBA+20g/L sucrose as the medium had a better multiplication effect with the proliferation coefficient 4.52.4.The strong seedling and root culture of Acacia fimbriata.The strong seedling medium was MS+0.2 mg/LIBA+15g/L sucrose.According to the rooting situation,the better medium was 1/2MS+0.2mg/LNAA+0.2mg/LIBA+15g/Lsucrose,with the rooting rate 99%.5.The transplanting of Acacia fimbriata.Combination of loess and peat soil with a ratio of 2:1 was used for transplanting,and the survival rate was over 89%.Based on the experiment of the effect of different transplanting time on the tissue culture seedling,the survival rate of transplanting reached the highest in May.