Effects Of The Immune Function And Endocrine Of Dermatomycosis In Cows

Dermatomycosis was chronic, local mycotic dertmatitis caused by dermatophytes with characteristics of depilating, scaling, commonly referred to as"Ringworm". The cows were easily to be infected because of its worldwide and strong infectious, especially in the dairy farm with poor management. Human beings also could be infected by contacting with the cows so we should pay more attention in the public health. There were many factors to cause the disease such as improper use of antibiotics, corticosteroids and immunodepressant. The incidence of dermatomycosis was increased in China in recent years. The growth of the cow would be seriously affected and the milk would cause serious contaminated with the disease.Studies of pathogens were concentrated for the dermatomycoses at home and overseas nowadays. Studies on the immune response and endocrine function of anti-fungal infection were fewer. Pathogenesis mechanisms were investigated by the isolation and identification of pathogens and the studies of endocrine function and immune response to provide theoretical basis and the prevention and treatment for the dermatomycosis.Samples were collected from the sick cows in Heilongjiang Province dairy farm and the blood smear and serum were got at same time. Samples were cultured in Sabouraud medium for isolation and purification. Blood was analysised by general method and leukocyte classification by Wright staining. T lymphocytes were detected by esterase staining. The CD4, CD8, M-CSF, contents in serum were detected by ELISA and the IL-2, IL-6, T3, T4 were detected by RIA. IgG, IgM, IgA levels were detected by turbidimetric method.The results showed that verrucous Trichophyton mentagrophytes was the main pathogens for cow dermatophytosis. The neutrophil, monocyte, and lymphocyte were significantly high in the sick cows and other hematological indicators did not change significantly.M-CSF and CD8 levels in the blood had significant difference (P <0.05). CD4/CD8 ratio was significant lower to compare with the control (P<0.05). IgG in the experimental group was significantly higher, IgM, IgA no significant changes.Thyroxine T3 and T4 were detected and showed the T4 levels were significantly increased in the sick cows while T3 without significant change.It was suggested from above that body fluids, endocrine and immune system had changed in sick cows. There were both specific immune response and non-specific immune response in the cows with fungal infection. There were also immune imbalance and endocrine disorder when the cows had fungal infection.