| With the development of people's living standard,higher wheat quality is demanded. Wheat seed storage protein and starch have strong effects on wheat processing character. Researchers have paid great attention on the researches of seed storage protein but starch component which is 70%of seed endosperm for a long time.Therefore,comprehensive understanding of the accumulation regularity of starch components and the effect of starch content on wheat quality are helpful for improving of wheat quality.Rye(Secale cereale L.) is closely related to wheat by taxonomy.It carries a great number of resistance genes and increasing yield genes.As bridged tool to translocate rye genetic material to wheat genetic background,Wheat-rye addition line plays a key role in wheat breeding,nearly 30 years.In order to study on the effect of different rye chromosomes on the expression of seed storage protein,choosing wheat-rye addition lines which have different genetic background is helpful to utilized rye further.For suppress interference of seed storage protein and defined the effect of starch components content on wheat quality clearly,five wheat sister-lines(J655,J210,J309-2,J285和J724) were used to analyze the seed storage protein and then choose the lines which have the same storage protein component to study the starch component accumulation regularity and correlation relationship between contents of starch component and wheat quality trait.In addition,recipient common wheat "Holdfast","Chinese Spring"; donor rye "King","Impire";their amphidiploids and addition lines which total is 8 were used to analyze their seed storage protein.Then,explored the effect of different rye genomes and chromosomes on the expression of seed storage protein in wheat genetic background.The main results are as follows:1.HMW-GS of all sister line were analyzed by SDS-PAGE.J655,J309-3,J210 have the same HMW-GS electrophoresis pattern,which is null,7+8,2+12.Two allelic variants(7+8 and 14+15)at Glu-1B locus in J724.In J285,there are two allelic variants(1 and null) at Glu-1A locus and two allelic variants(2+12 and 5+10)at Glu-1D locus.There are no differences among the gliadin forms of J655,J309-2 and J210.There are two banding forms inα,γandωregion respectively in J285.J724 have two banding forms inα,γandωregions respectively.In addition,there is one lane which have different mobility band from others inβregion.It is showed the HMW-GS components and gliadin forms have to still not be homozygous even if in advanced wheat lines and we must pay more attention to chose from advanced wheat lines.2.The results showed there was no significant different accumulation regularity of starch components among the 3 wheat sister-lines.Amylopectin had much higher accumulation rate than that of amylose at middle and late grain filling stage.J655 had the highest amylose content,the lowest amylopectin content and the smallest ratio of amylose content to amylopectin contents.J309-2 was contrast to J655 in the traits of starch components content and the ratio of amylose content to amylopectin content.The results showed there is no significant differences of starch components accumulation regularity among sister-lines while the obvious differences of starch component content was found among sister-lines.Therefore,we can management water and fertile through late grain-filling stage to regulate the accumulation of starch component and that work to breed wheat for specialized.3.The results of correlation analysis of starch components content and wheat quality trait showed:(1) The amylose content were highly significant negative correlated to the contents of amylopectin and starch while were significant correlated to the ratio of amylose content to amylopectin content.(2) The amylose content was significant correlated to the falling number and the gluten index.The amylopectin and starch content were highly significant correlated to gluten index.(3) The amylose content and the ratio of content to amylose content to amylopectin content were significant correlated to water absorption while the content of amylopectin and starch had significant correlation to water absorption. The results showed amylopectin content has obvious effect on starch content and has favorable influence on gluten quality.So possibility,amylopectin content is a good tool to estimate gluten quality.4.Holdfast-King,Chinese Spring-Impire wheat-rye alien addition lines and 2 wheat-rye amphidiploids were separated by SDS-PAGE and A-PAGE and the results showed:(1) The HMW prolamin in 2 amphidiploids all presented the same banding pattern to wheat parent. In addition,amphidiploids presented a band which had different mobility from parents. The gliadin forms in amphidiploids showed codominance.(2) There were three patterns of HMW prolamin identified in Holdfast-King addition lines.1R addition line showed the same HMW prolamin pattern to amphidiploid as well as its gliadin form.2R,4R,5R,6R, 7R addition lines all showed the same HMW prolamin to recipient parent which contain 5 bands while 3R addition lines had only 4 bands and a band had different mobility from amphidiploid and the other addition lines.Similarly,2R,4R,5R,6R and 7R addition lines had the same gliadin form to recipient parent,but 3R disappeared 2 bands inω-region.(3) The banding patterns of HMW prolamin in 1R addition line of Chinese Spring-Impire overlapped those of its parents.But its gliadin form expressed the banding pattern of recipient parent as well as the rest addition lines(from 2R to 7R).The wheat genome and the rye genome should take effect on each other in amphidiploid,which could have influence on the expression of genes controlling the seed storage protein.And different rye chromosomes which are in the same rye specie might take different effects on the seed storage protein expression.
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