| Hybrid Cymbidium is a new variety which derived from Cymbidium hybridum and Cymbidium faberi. Cymbidium hybridum is popular to native gardening because its flowers are big and solemn and flower season last long. Since selected parents for crossbreeding lack fragrance gene, descendant nurtured often do not has fragrance. But the Chinese feel minor defect in something otherwise perfect unavoidably because they are accustomed to appreciate'color, fragrance and flavor'of Chinese Cymbidium. Hybrid Cymbidium partly is Cymbidium hybridum with fragrance, which has big and colorful flowers of Cymbidium hybridum and the fragrance of Chinese Cymbidium. It makes up Cymbidium hybridum's shortcoming of without fragrance, so it has very good ornamental value and market prospect.The germination of Orchid's seed is difficult under natural condition, and seeding can hardly inherit good characteristics. Traditional cultivation depends on minute reproduction, but the reproduction cycle is long and reproduction rate is low, which can not meet the needs of large scale production. Protocorm like bodies are induced from shoots of hybrid Cymbidium by tissue culture, and they can easily proliferates massively, so we can obtain large number of plantlets with uniform characteristics. This is the best way of industrialization production of Hybrid Cymbidium. Hybrid Cymbidium is different from Cymbidium hybridum and Chinese Cymbidium. And the study on the technology of its tissue culture has not been found yet.The shoots of hybrid Cymbidium were used as explants to establish a rapid technique system of micropropagation, and to study the suitable medium for propagation and differentiation of PLB, and the proper culture method. The results were as follows:(1) Temperature for culture: Day 26℃,Night 24℃. 12 hours illumination every day, and about 2000 Lux illumination intensity, 75% relative humidity.(2) The inducement medium of the PLB:MS+6-BA1.0mg·L-1+IBA 0.5 mg·L-1+AC 3.0 g·L-1. After 30 days culture, the inducing rate of the PLB could reach 100%.(3) The proliferation of the PLB: MS+6-BA 0.5 mg·L-1 +IBA 0.1 mg·L-1 +AC 1.0 g·L-1 was the most suitable medium. After 15 days culture, the multiplication rate of the PLB could reach 3.4.(4) The differentiation of the PLB:1/2MS+6-BA 1.5 mg·L-1 +NAA0.5 mg·L-1 was the best medium.Each explant can obtain 3.2 shoots averagely.(5) The optimized condition of proliferation of the PLB:the best AC density is 1.0 g·L-1, the best sugar concentration is 30 g·L-1, 0.3cm×0.3cm is the best explant size,and best abstance is 2.0 cm each other.(6) Seedling stage: the medium for the elongation of shoots and leaves was 1/2MS+6-BA1.5 mg·L-1 +IBA0.05 mg·L-1 +AC0.2 g·L-1; the medium for the rooting of shoots was 1/2MS+6-BA0.5 mg·L-1 +IBA0.05 mg·L-1 +AC1.0 g·L-1。(7) Active carbon was effective for preventing the explants from browning to a certain extent, 3.0g·L-1 was the suitable concentration for inducement, and 1.0 g·L-1 was the best concentration for the PLB's proliferation.(8) For gardening of plantlets, the soil of the mess was the suitable material. The survial rate of plantlets could be up to 100%.
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