The Study On Mutagenic Effects Of ⁶⁰Coγ-ray On A.belladonna.L

Hippeastrum vittatum(Amaryllis vittata )is one of the tuber flowers,which belongs to Hippeastrum Amaryllidaceae.It is a perennial herb.Tts bulb's shape is like an egg,and its diameter is about 7-8cm。Hippeastrum vittatum(Amaryllis vittata ) is produceed in the South America,Peru,Brazil originally,which is planted widely in various countries now.Tts flower is big and colorful,and its leaves are deep green which seem to be always flesh.Hippeastrum vittatum(Amaryllis vittata) is suitable for outing flower and bonsai watching.It may also can be disposed in the garden,the flower-bed,the flower-strewn path and the forest with nature arrangementoIn this experiment,three-year-old bulbs of Hippeastrum vittatum(Amaryllis vittata) were radiated by ⁶⁰Coγ-ray with different dosages(5 Gy,10Gy,15 Gy,20 Gy).And established the control group.at the same time,Carry on the field observation to its survival rate and the seedling character.The results indicated that: along with the Increasing exposure dosages,the Survival rate,lengths of the leaves,the widths of the leaves and so on were decreasing progressively.Analysis the leaves,which were radiated by ⁶⁰Coγ-ray with different dosages,by the PAGE.The results indicated that:Under the 5Gy exposure dosage,the activenesses of the peroxide enzyme and esterase were the strongest。The above results are provided the basis for the further study on Hippeastrum vittatum(Amaryllis vittata)。The research indicated that:⁶⁰Coγ-ray caused the multi-position mutagenic effect.on the bulbs of Hippeastrum vittatum(Amaryllis vittata).the first step of this experiment was to transplant them in the simple protected fields after radiated in January.the second was to survey different agronomic characters of the targets when they were pulling out the leaves and blossoming..The results showed that:along with the Increasing exposure dosages,the Targets of the injury tolerance were increased in the M1 generation,such as the survival rate,lengths of the leaves,the leaves the widths,the leaves,the main root quantity,the Inferior root quantity and so on..they could mainly be displayed in the following several aspects:Along with the Increasing exposure dosages,the survival rate reduced obviously.The group radiated by 20 Gy diesd completely.â‘¡lengths of the leaves were shorten remarkably.the mean value of the leaves lengths in the 15 Gy dosage radiation group was about 40% of the control group.â‘¢along with the Increasing radiation dose,The widths of the leaves did not change obviously.The variance analysis indicated that the results were not remarkable;â‘£along with the Increasing radiation dose,The quantities of the main root,and the inferior root reduced obviously;⑤along with the Increasing radiation dose,The lengths of the main root,and the inferior root shorten obviously. This was also the two the primary causes of the t adult plants which the growing trend weakened unceasingly.By processing the the obtained data through the variance analysis and the SPSS software,the following equations could be Obtainsed separately::The relationship between different dosages and the lengths of the leaves was fitted in the dependent equation: y=-0.405x+9.04 R²=0.93 F=54.291＞F_0.01=34.12The relationship between different dosages and the widths of the leaves was fitted in the dependent equation:: y=-0.129x+3.306 R²=0.68 F=9.484＜F_0.05=10.13The relationship between different dosages and the quantityies of the main roots was fitted in the dependent equation:: y=-0.362x+6.876 R²=0.924 F=49.551＞F_0.01=34.12The relationship between different dosages and the lengths of the main roots was fitted in the dependent equation:: y=-0.37x+7.502 R²=0.995 F=758.981＞F_0.01=34.12The relationship between different dosages and the quantityies of the inferior root roots was fitted in the dependent equation: y=-3.518x+61.728 R²=0.868 F=27.378＞F_0.05=10.13The relationship between different dosages and the lengths of the inferior root roots was fitted in the dependent equation: y=-0.126x+2.624 R²=0.989 F=365.095＞F_0.01=34.12 X was Independent variable(dosage)in the above dependent equations. Y was the target value.with the corresponding agronomic characters.Radiation Unit of the root tip cells chromosome compression,observed that,5 Gy treated the frequency of chromosome bridge for 36 percent,10 Gy team chromosome fragments in the frequency of 33 percent,to verify the radiation caused by chromosome mutation major structural variation the main conclusions.Guaiacol with the M₁ and the survival of plant leaves for peroxidase analysis, using naphthalene acetic acid ester of the M₁-generation plant leaves for survival EST analysis,the results are as follows:the control group treated relatively peroxidase Has increased the number of bands,with the control group A,5 Gy-treated group increased to A band,B band,C band,10 Gy-treated group increased to A band,B band.Once again proved peroxidase sensitivity to outside stimuli,the group handling the increase in the number of different bands,may mean different kinds of plants outside stimulate different responses,5 Gy treated the largest number of bands,this means that the plants on the dose response Strong in dealing with this dose,to effect greater than lethal effects.5 Gy team EST significantly wider bands,10 Gy team EST bands were significantly weaker,but no new bands increased,once again proved the stability EST.Isozyme bands and the availability of power to reflect changes in ⁶⁰Coγ-ray radiation dose effect,and characters with a certain degree of injury related. Therefore,the isozyme can be used as ⁶⁰Coγ-ray aider the biological effects of radiation detection of a chemical or biological indicators.