Microsatellite Polymorphism For Three Breeds Of Sheep And Hybrid Offspring

The genetic diversity for Poll Dorset,Sall Tail Han sheep,Mongolian sheep and their hybrid offspring was studied using 12 microsatellite DNA markers(McM42,MAF65,OarFCB11,McM527,HSC,OarFCB20,OarAE101,RM0509,MNS0094,ILSTS004,BM1329,CSSM0004).The genetic heterrozygosity(He),polymorphism information content(PIC),dominate alleles frequency,Nei Standard Genetic Distance and cluster analysis were determined to evaluate the genetic vaviability within breeds(populations) and genetic relationship among breeds(populations).The results were as follows: The 12 microsatellite loci for 125 blood samples of the 5 populations were analyzed by vertical non-denatured popycrylamide gel electrophoresis(PAGE) figures.There was abundant popymorphism in 12 lozi,198 allels in total are detected,the mean of each locus was 16.5.The polymorphism information content(PIC) of each site was abundant in 5 populations ,the minimum PIC was 0.7825;The genetic heterrozygosity(He) for Poll Dorset,Sall Tail Han sheep and Mongolian sheep was 0.8642,0.8558,0.8425 respectively;The maximal Nei Standard Distance(0.2327) among them was between Poll Dorset and Sall Tail Han sheep,the follows in order were respectly Poll Dorset and Mongolian sheep, Sall Tail Han sheep and Mongolian sheep.The microsatellite loci analysis showed that Poll Dorset and Small Tail sheep had the furthest relationship.The relationship was the closest between Small Tail sheep and Mongolian sheep.The perfect project for New Mutton Sheep Breed in the north of China at Microsatellite DNA molecular level is that using Poll Dorset as male parent and SmallTail Han sheep and Mongolian sheep as female parent.