Studies On Antagonism Of Taurine On Maduramicin Toxicity

Maduramicin is a polyether ionophore antibiotic widely used against coccidiosis in veterinary practice, but it can caused severe toxicosis in domestic animal. Polyether ionophore antibiotic toxicosis is difficulty to diagnosis and no potent detoxicant is available now. The Research goal is to study on maduramicin toxic dose in rats and explore the protective effects of taurine on maduramicin toxicosis.1. toxic dose by maduramicin in rats: 48 SD rats were randomly divided into six groups, 8 rats in each group. Three dose groups were orally given maduramicin by orally at dose of 32, 16 and 8mg/kg b.w/d respectively, and one control group. The others were two multiple exposd groups in order to evaluate the toxic effect of multiple exposure, which were given maduramicin twice and thrice respectively. The clinical symptoms were observed on maduramicin exposed rats. All the rats were euthanized when rats were administrated 12 hours later, and gross lesions were observed in tissues. SOD and MDA were tested in serum, liver, myocardium and thigh muscle, and GSH-Px was also tested in serum and liver.With the exposed dose increasing, the clinical symptoms such as anorexia and lethargy became more obvious in exposed rats. The hind limb paralysis or even dead could be observed when rats were seriously in intoxication. Pathological changes were obviously observed in liver and myocardium. The contents of lipid peroxidation production increased and the activity of antiperoxidase decreased after administration in rats, and the lipid peroxidation damage was more serious with the increasing dose of maduramicin. The results of multiple exposures showed that maduramicin has cumulative toxicity. As the clinical intoxicated symptom of rats, the determined index in tissue and the number of dead rats, the fine toxic dose could be induced by maduramicin was administered two times by 8mg/kg b.w in rats.2. the protective effects of taurine in maduramicin intoxicated rats: 40 SD rats were randomly divided into 5 groups: two contral group, three treatment groups. The rats were adminstered maduramicin at a dose of 8 mg/kg b.w./d for 2 days. Before maduramicin exposure, three treatment groups were orally given at a dose of 1%, 2% and 4% taurine solution for 15 days, and continually given by taurine solution during maduramicin administration. The clinical toxic symptoms were observed after administration of maduramicin. After maduramicin expouse 12 hours later, all rats group were euthanized, and gross tissue lesions were observed. SOD and MDA were tested in serum, liver, myocardium and thigh muscle, and GSH-Px was also tested in serum and liver.The results showed that taurine could decrease the contents of the lipid peroxidation production, increase the activity of antiperoxidase in maduramicin exposed rats, and relieve the clinical toxic symptoms. The antioxidation effects of taurine were related with dose. The above results suggested that taurine by drinking at doses of 2% ~ 4% could provide good protection for tissue damage induced by maduramicin.