Nitrogen Ion Implantation Induced Sophora Alopecuroides Total DNA Transformation Into Yeast

Medicinal plant Sophora alopecuroides,containing many natural products such as alkaloids, flavonoids, organic acids, amino acids, protein and polysaccharides, is of important physiological function and pharmacological effect, especially the effect of alkaloids are significant in treatment of hepatitis b, antitumor, health care, prevention and control of plant diseases and elimination of pests and so on, also it has a wide application prospect in medicine, pesticide science and food preservation. Therefore, it is of great significance to explore other ways to obtain the natural products of Sophora alopecuroides. In this study, flavonoids and alkaloids of whole-plant Sophora alopecuroides were prepared by applying conventional methods; Total DNA of wild Sophora alopecuroides were prepared and purified by CTAB, also better extraction way of total DNA of wild Sophora alopecuroides were achieved; Firstly studying on nitrogen ion implantation induced the random transformation of Sophora alopecuroides total DNA into four kinds of yeasts by using platform of ion beam mediated transgene technology, and 715 recombined yeast strains of T2 generation were obtained. 114 recombined yeast strains of T3 generation, including 21 strains of positive responses about flavonoids substances with hydrochloric acid and Mg, 65 strains of positive responses and 28 strains of abnormal response about the alkaloids substances with phospho-molybdic acid and bismuth potassium iodide, were obtained through the quantitative analysis of flavonoids and alkaloids. One kind of metabolites of 3 recombined yeast strains contained the same Rf value of flavonoids substances of Sophora alopecuroides and the metabolites of 1 recombined yeast strains contained four kinds of flavonoids substances were detected by TLC, It had made some beneficial exploration for low-energy ion implantation induced Sophora alopecuroides total DNA macromolecule transformation into yeast. Recombined yeasts which were obtained in this study can provide the valuable materials to further study for chemistry evidence and molecular evidence of low-energy ion implantation induced Sophora alopecuroides total DNA macromolecule transformation into yeast.