Preliminary Study On Bamboo Seed Callus Induction And Different Antioxidant Effects Of Browning

Phyllostachys heterocycla(Carr.) Mitford ' Ventricosa is a grass family Phyllostachys,a single bulk-type,evergreen plants. Bamboo area is the most widely cultivated,bamboo is the most important economic value.This article mainly from the active characteristics of bamboo species of embryo, embryo of callus induction,callus browning prevention treatment of the three aspects for research.In bamboo embryo as explant,mainly around the kind of seed soaking time and temperature of embryo,embryo of disinfection sterilization method,the best medium of callus type and ratio of hormone,different anti browning callus proliferation and the effect of the treatment methods of inhibiting browning phenomenon such as a variety of problems. Research data in the process of using EXCEL software and SPSS 21.0 statistical software processing,obtained the following main results:1 ? The main factors influencing the bamboo seed germination are: seed soaking temperature and seed soaking time.The best seed soaking temperature is 35 ?,the best seed soaking time of 5 h.2?Bamboo seeds best disinfection way for: 75% alcohol soak 4 min ? Sterile water flushing 6 times ? 2% Na Cl O soak 12 min ? Sterile water flushing 6 times ? 0.2%Hg Cl2 soak 15 min(3-4 drops of twain- 20)? Sterile water flushing 6 times ? Blot the excess water ? vaccination.3?The main factors influencing the bamboo kind of embryonic callus induction: Kind of embryo vaccination way, the basic culture medium type,cytokinins 2,4-D,ZT. Removal of most of the endosperm,embryo and embryo with groove side down,placed under the precondition of vaccination,The optimal culture medium bamboo kind of embryo callus induction type is : MS + 4. 5 mg/L 2,4-D + 0.2 mg/L ZT + 8g/L Agar + 28g/L sucrose,p H=5.8.4?In adding a certain kind of embryo callus induction medium concentration of ABA can effectively suppress together with callus induction of adventitious bud and adventitious roots,and can improve callus morphological characteristics. The best ABA concentration is2 mg/L.5?The main factors influencing the bamboo kind of embryo callus proliferation are:the basic culture medium type,cytokinins in 2,4-D and 6- BA and auxin NAA. Bamboo types of embryo callus proliferation the optimal culture medium is:MS + 2. 0 mg/L 2,4-D+ 0.5 mg/L NAA + 0.1 mg/L 6-BA + 8g/L Agar + 28g/L sucrose, p H=5.8.6?Prevent browning in processing can effectively curb of embryonic callus browning main measures are : kind of embryo pretreatment, adding antioxidants,control of light intensity. The vaccination before with 100 mg/L cysteine to soak, kinds of embryo culture medium added 10 mg/L ascorbic acid,in dark conditions after inoculation training can effectively inhibit browning phenomenon to appear.On the whole,in this paper,on the one hand to say,the bamboo seed activity characteristics,select the most suitable for seed germination of soaking temperature and time, the inoculation of seed way,etc. On the other hand,the bamboo seed sterile to establish the system of rapid propagation and induction of embryonic callus of the best hormone combinations, and select effective way to prevent browning. So at this stage the bamboo is the basis of comprehensive sequencing,can provide efficient bamboo callus conversion rate,for the works carried out smoothly at later stages of bamboo genetic engineering of appropriate materials,has important significance in the genetic breeding.