| 'Cherry', 'Fortodi', Ben Nevis', 'Bona', 'Hanfeng'and'Risager 'were used as matierals to be syudied on the technique system of tissue culture and rapid propagation in this paper. Five stages were involved in the whole technique system, including establishment of clone system, multiplication of shoots, nurture seedlings, induction of callus and rooting. The clone systems in currant shoot of 'Cherry', 'Fortodi', 'Ben Nevis','Bona', 'Hanfeng'and'Risager' were established for the first time , which would be the basic of further research by means of biotechnology in the future. The results are shown as below:1 The healthy one-year-old branch of currant were selected for water culture, after the cold sand of dormancy. The tender tip of Ribes were used as explant. It revested that soaking explant by HgCl2(0.1%) for 5min. The survival rate can reach 80%. the quality of the shoot is different when the different medium were used. 1/2 MS + IBA 1.0 mg·L-1 for the medium, producing the stem diameter and height in vitro significantly in MS +6- BA 1.0 mg·L-1+ IBA0.1 mg·L-1 produce processing plants, and plant growth robust.2 In the process of the currant cell proliferation, BA1.0 mg·L-1+IBA 0.1 to 0.2 mg·L-1+MS formula is the easiest most ideal training effects.The bud proliferation multiples of 'Hanfeng'and Fortodi' can be achieved four times.3 Several formulations of rooting contrast effect, which is the best rooting ,1/2 MS+ IBA1.0 mg·L-1 rooting rate reached 100%. The shoot grow strong, and leaves are dark green.It is conducive to the conduct of plant photosynthesis stored organic matter. Plant Festival which clearly subculture simple. Multi-branched root, root level index could reach 4, absorbing roots more easily absorb water and nutrients. Rooting the number of days in the control about 30 suitable, the length of seedling root growth of the greater degree of lignification impact subculture seedling survival rate.4 The currant tissue culture proliferation of cultured Plexiform was no festival, and the separation more difficult. This test to take alternate training methods, using MS + BA1.0 mg·L-1 and 1/2 MS + IBA1.0 mg·L-1 two medium culture.It can decrease the separation operation of inconvenience. At the same time a large extent, to avoid the same kind of plant hormones in the body caused by the large number of residual inhibition. From the height and diameter, and other aspects of the investigation and a simple differentiation of the plant was a very significant difference.5 The content of the SOD,POD in vegetative organs,that marks the strength of its metabolic activity. And also shows that the plants vegetative organs dedifferentiation capacity. Currant test measured vegetative organs and POD,SOD content from more to less as a leaf> petiole> Root> stems and content and the difference showed significant differences. Currant organ dedifferentiation capability is weak, so test taken two steps induced spike currant will be the first training in the petiole MS + BA1.0 mg·L-1+2,4-Dmg·L-1 medium,15 days later transferred to the MS +1.0 mg·L-1 + NAA 0.4 mg·L-1+ AC 1.0 g + AgNO3 1.0 mg·L-1 medium.The callus is green larger. The currant Callus browning phenomenon more serious, from the perspective of cytology brown callus observed that the majority of non-nuclear, cytoplasmic minimal, as were many red cells in the spherical body, the cells are loose, shape gradually incomplete.6 The stems of currant in MS + BA 1.0 mg·L-1+IBA 0.1 mg·L-1, the base of its stem dedifferentiation the callus mass. After a period of time cultivate, It can differentiation new growth of shioot. Observation by paraffin sections plants not found between the catheter and Cypriot connections, the new plant is an independent body. Callus also found that the presence of many mass Bud primordium.
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