Genetic Linkage Map And Quantitative Trait Loci Controlling Vegetative Growth Rate In The Edible Basidiomycete Flammulina Velutipes

Mating type of 120 single spore isolates (SSIs) from fruiting bodies of (Flammulina velutipes (Fr.) Singer) were identified. Based on this, 107 dikaryotic strains of F2 mapping population were constructed. Based on law of independent assortment between color gene and mating gene, 3 recombination strains with c gene and A6B6 from monokaryotic strain Sanming hu tou san wild and Gold 21 were generated. One of them, monokaryotic strain No.77, with well growth rate, as tester was paired with SSIs. Testcross population was generated.FL19 protoplast monokaryotic strain L11,8801 protoplast monokaryotic strain W23 , and the hybrid strain 1123 were used as templates in PCR to select primers which can produce polymorphic bands between two parental monokaryons. In my research, 127 polymophic bands were produced, 108 of them originated from RAPD, 19 of them originated from SRAP. 81 polymorphic bands were sequenced. 25 polymorphic bands were converted into SCAR molecular marker successfullyA genetic linkage map of the edible mushromm Flammulina velutipes was constructed by molecular marker and phenotype marker, with software Mapmaker 3.0.Based on SSIs mapping population using the Backcross data type, 45 markers including 42 SCARs, 2 mating factors (matA and matB) and fruiting body color, were grouped. 35 markers were linked in 8 linkage groups. The length of the 8 linkage groups ranged from 31.1cM to 269.1cM, and they covered a distance of 785cM, and the average distance of the interval between adjacent markers was 29.0cM. The other genetic linkage map was constructed based on F2 mapping population, using F2 data type. 43 markers including 42 SCARs and fruiting body color were grouped. 36 markers were linked in 11 linkage groups. The length of the 11 linkage groups ranged from 23.8cM to 193.5cM, and they covered a distance of 558.1cM, and the average distance of the interval between adjacent markers was 21.5cM.The SCAR marker, S10₇50, linked to the matA gene was identified. It was located in No.1 linkage group. The distance between S10₇50 and matA gene was 20.9cM.The frequency of traits including mycelium growth rate, production, the length of stipe and the size of cap showed continuous variation, according with the character of quantitative trait. We analyzed QTL by software Network-2.0. The QTL(qMGRS1)controlling the growth rate of monokaryotic mycelium on straw-based medium was identified, accounted for the 13.8% of its viriation. The two loci (qMGRS5_i and qMGRS8_j) involved in epistatic interaction, were identified. Based on testcross population, the two loci (qMGRP2_i and qMGRP3_j) involved in epistatic interaction, effecting dikaryotic mycelium on PDA medium were identified. QTLs controlling other traits weren't identified.