Identifination Of Mushroom Germplasm Resources And The Preservation Methods

Comparison of the current market chaos mushroom species, strain lags behind the management, indiscriminate introduction, naming chaos more common, resulting in the same homonym or synonym is more serious, is not conducive to mushroom varieties the objective to reveal the genetic diversity. Identification of mushroom strains of bacteria to protect resources and prevent degradation in bacteria is also of great importance. In this paper, reference to the basis of relevant information both at home and abroad, combined with DNA molecular markers and the antagonist experiment research in this area.In this paper, on the ITS-PCR amplification to obtain 136 species of mushroom cultivation for DNA extraction to verify the successful screening of the eight pairs of SCAR primers, SCAR primers in authentication, the bands showed polymorphism. Through 8 SCAR molecular markers to 136 species of cultivated mushrooms and a cluster analysis of genetic relationship, the result is divided into 49 categories, of which 20 types of composite-type, single-category 29 category. Antagonism at the molecular clustering experiment is carried out on the basis, divided into three types of situation, they are: complex molecular clustering for the same type of antagonism between the molecular clustering for different types of antagonism between the molecular phylogenetic relationship clustering distant between the type of antagonism. The result is the existence of various types are not inter-antagonist and antagonist, through experiments that the antagonism of the classification and molecular markers linked classification of both the difference and can be all into the system. Bred a new strain, due to factors such as physiological and ecological out-of-control, resulting in degradation or aging of bacteria, a direct impact on the quality, resulting in the failure of cultivation. So often the problem caused by bacteria disputes, and even lawsuits. In order to facilitate the reduction of bacteria and aging degradation, mushrooms Culture Collection experimental study is particularly important. In this paper, six single-factor-based tests preservation experiments are: the study of different preservation methods, the study of different preservation media, cooling study different procedures, different strains of TSE research, the study of different thawing temperature, different antifreeze研究. The results of screening for the best conservation program in order to select the wood for the young medium of mushroom preservation, anti-freeze to 10% of glycerol, the use of cooling procedures for instrument cooling (from 4℃to -40℃in order to cool the rate of 1℃/ min most good) to the liquid nitrogen filling preservation, the preservation removed after 25℃-35℃in the water bath to thaw activation. Preservation process in which different cooling or storage in the first case of mushrooms. Through the test of mushrooms Culture Collection also played a certain reference value.Different methods of preservation in order to verify the growth rate were determined after the results of its determination to carry out the activity test, were determined by laccase, CMC enzyme, xylanase, amylase enzyme activity curves of four kinds. Seen from the results of different strains of different preservation methods have these types of enzymes, and the curve measured after preservation in different ways and measured growth rate was positively correlated, showing a certain degree of regularity. This is not literature in other flies, but also preservation of mushroom strains to provide a basis for the physiology and biochemistry.