The Polymorphism Of FSHβ Gene And Their Relationship With Fecundity Traits In Goats

Follicle-stimulating homone(FSH) is a pituitary glycoprotein hormone.FSH is ctitical hormones to follicular growth,development.,dominace,maturation and ovulation.They also influence the growth of the reproductive system and the quality of sperm.The polymorphisms of goat FSHβwas detected by PCR-SSCP method with different pairs of primers among 33 goats among 166 Tianfu goats,101 Boer Goats and 67 Chengdu Brown goats(Acquisited by Sichuan Agriculture University and Huiyuan Animal husbandry Company).In this study,Comparing the FSHβof sheep,bovine and pig in Genl3ank,the primers were designed and the 5' regulating region,the first and second exon of different goat breeds,are amplified.There are one SNP sites in the 5′regulating region of FSHβgene,Other two primers have no SNP site.Ii is A712G.T.The primers 1 have a middle PIC(0.25＜PIC＜0.5) in Tianfu goat.but have a lower PIC(PIC＜0.25) in Boer Goat and Chengdu Brown goat.The result of x2 fitness test of primer 1 indicated that Boer Goat was hightly significantly biased from Hardy-Weinberg equilibrium(P＜0.01),but Tianfu goat and Chengdu Brown goat are all kept in Hardy-Weinberg equilibrium(P＞0.05).Among goats with littering records,the least squares analysis showed that:the litter of AA genotype in goat is more.0.39(P＜0.05) than BB genotype,The litter of secong kidding in goat is more0.6 than that of first kidding in goat(P＜0.05);The litter of third kidding in goat is more0.87 than that of first kidding in goat.(P＜0.05).The litter is most in third kidding with AA genotype in Chengdu Brown goat.It is up to 2.60 per kidding.The effect of AA genotype in second and third kidding is stronger than that in first kidding.(P＜0.05).The litter of AA genotype is more than BB genotype by same kidding of any kind of goats.Data obtainedni this expriment indicated that the polymorphism of fragments amplified by one pair of primer probably associate with goats productive trait,but need further studies.