| The Chive (Allium schoenoprosum L.) is a perennial herbaceous plants,belonging to the family Liliaceae. Stem disc explants for the establishment of tissue culture chive technical systems was studied. The study was focused on sterilization of explant system, parts of explants, chive hormones on root induction of adventitious buds and impact, Regeneration System of Chive have be established.In this study, 15 sterilization methods were used. The results showed that 0.1%HgCl2 (Tween 20) , 0.1% HgCl2 (Tween 20) and 2% NaClO, as well as with Streptomycin Sulfate ,had no effect. However, extention of the sterilization period of 0.1% HgCl2 (Tween 20), resulted in gradual increment in the death rate of stem discs. Increased penicillin in the Induction medium reulted in the suppression of growth of endogenous fungi, up to the density of 400 unit /L.The size of stem disc play an important role in bud induction. Stem discs too small (independent stem disc) or oversized (stem disc adds leaf base 0.2 cm) could not effectively achieve the multiplication the goal. Therefore it is important that a suitable sized stem dics are chosen. In this study stem disc of size 0.1cm produced the highest number of buds, which happened to be an intermediate size between the too small and the over sized stem discs.Comparing the effect of the hormones, results showed that 6-BA played a principal role than NAA in bud induction. Elevating the density of 6-BA resulted in the elevation of the Vitrification frequency . Without NAA in the culture medium stem dics could be induced to produce adventitious buds. However an increase in the density of NAA in the culture medium is better than without and could moreover reduce the vitrification rate effectively. Finally the most induction rate was observed in medium containing hormone densities of 2.0 mgL-1 and 0.2 mgL-1 for 6-BA and NAA respectively with average number of induction of 13.73. Comparing the three kinds of basic media (MS,LS,B5) ,the results showed that induction of adventitious buds on three kinds of culture medium differed. MS is the best medium for effective bud induction, this is because of the presence of NO3--N and Vitamin in it which are absent from the other two media.When the adventitious buds grew to 1.0 cm (vaccinated 20 d) , they were quickly placed in separate media with /without root induction hormone. The adventitious buds rooted on medium without NAA ,but more roots were produced when buds were placed in a medium containing NAA. However, when NAA density was increased to 1.0 mgL-1, the root turned yellow, this will not favor transplanting. In this study the best density for NAA is 0.5 mgL-1.After the buds have developed roots about 20days, they were transplanted to the four different kinds of matrices determine their survial rate. Comparing four kinds of matrices ,results showed that soil (soil: peat =1:1) was the best of the matrices used with survival rate of 83.33%. |
