Single Nucleotide Polymorphism Of TCAP,DECR1,PRKAG3 In Yanbian Yellow Cattle And Its Association With Meat Quality Traits

The polymorphisms of TCAP.DECR1/PRKAG3 gene were detected by PCR-SSCP method in Yanbian Yellow Cattle, and to reveal the association between SNPs and meat traits. The resuls showed that:(1) Three mutational sites were founded in TCAP, which is 267 C-T mutation,300 T-C mutation,336 A-G mutation. Tow mutational sites were founded in DECR1, which is 23263 A-G mutation and 23473 A-G mutation. One mutational site was founded in PRKAG3, which is 4738 C-T mutation.(2)TCAP gene and PRKAG3 gene are in Hardy-Weinberg balance, and DECR1 gene is not. The analyzed of hereditary variation showed, the homozygosity, heterozygosity and effective alleles number of TCAP is 0.6322,0.3678, 1.5818, respectively. The homozygosity, heterozygosity and effective alleles number of DECR1 is 0.6922,0.3078.1.4447, respectively. The homozygosity, heterozygosity and effective alleles number of PRKAG3 is 0.6113,0.3887,2.5727. The polymorphism information content of these is 0.3013,0.2604,0.3051, respectively. They are midrange polymorphism.(3) The relationship of TCAP. DECRK PRKAG3 with meat traits was studied, the results is genotype AA of TCAP is negatived correlation with cooking loss, genotype AB of TCAP is positived correlation with c*2-1 (chroma). Genotype AA of DECR1 is negatived correlation with cooking loss and pH2·1·Genotype BB of DECR1 is negatived correlation with pH1-Genotype AB of DECR1 is positived correlation with L*, (lightness).Genotype AB of PRKAG3 is positived correlation with tenderness, and genotype AB of PRKAG3 is negatived correlation with a*21 (red) and b*24 (yellow).(4) The relationship of TCAP,DECR1,PRKAG3 with fatty acid content was studied, the results showed:genotype BB of DECR1 is negatived correlation with tetradecylic acid, and it is positived correlation with stearie acid and linoleic acid. Genotype BB of PRKAG3 is positived correlation with oleinic acid. TCAP gene have no significant correlation.(5) The relationship of TCAP, DECRK PRKAC3 with ami no acid content was studied, the results showed:genotype BB of TCAP is positived correlation with aminoacetic acid. Genotype BB of DECR1 is negatived correlation with histidine and lysine. Genotype AA of PRKAG3 is positived correlation with aspartate. Genotype AB of PRKAG3 is positived correlation with glutamate, alanine, praline, arginine, valine, respectively.