Correlation Analysis Of Microsatellite DNA Markers For Some Production Traits In Yanbian Yellow Cattles

To evaluate the genetic polymorphisms and to search for available molecularmarker for Yanbian Yellow Cattles, 30 Yanbian Yellow Cattles were genotyped with12 microsatellite markers. And the performance traits including the regulation of beefcattle linear valuation were measured, the association between genetic markers andperformance traits were analyzed by using general linear model (GLM). The resultswere as follows:12 microsatellite loci were used to analyze the genetic structure and geneticvariation in Yanbian Yellow Cattles. This study calculated the size and frequency ofallele and genotype frequencies, effective number of alleles, heterozygosity andpolymorphism information content. A total of 99 alleles were detected, with thenumber of alleles ranging from 6(BM1818) to 12(TGLA227), giving a mean numberof 8.25 alleles per locus. The total and mean effective allele number were 65.0652 and5.4221, respectively, with ranging from 3.5240～7.5358. The effective number ofalleles with six microsatellite loci HEL9, ETH225, BM1824, TGLA 126, BM1818 andTGLA122 were higher than other varieties of cattle. The heterozygosity were from0.7162(ETH104) to 0.8672(TGLA227), and mean heterozygosity were greater than0.5, all the experimental study sites rich in genetic, diversity. Polymorphisminformation content values ranging from 0.6795(ETH104)～0.8546(TGLA227). Allthe 12 microsatellite loci were highly polymorphic, which showed that there richgenetic polymorphisms and higher variability at these detected microsatellite loci inYanbian Yellow Cattles.The study found a total of 11 alleles of 9 microsatellite loci that had a positive ornegative relation on beef performance traits of Yanbian Yellow Cattles. Formicrosatellite locus BM1818, allele F (272) had positive relation with topline and forelimbs; For microsatellite locus TGLA126, allele B (114) showed positive, effects withskeleton and skin, and allele D (118) showed negative effects with topline, skeletonand skin; For microsatellite locus BM2113, allele G (138) had negative effects onhead and shoulder; For TGLA122, ailele E (147) showed positive relation with body diagonal length, head and skin; For SPS 115, allele B (247) had positive effects withskeleton; For HEL51, allele H (184) had positive relation with heart girth,left-forelimb girth, withers, shoulders, loin width, loin thickness, fore limbs, thighmuscularity and buttocks shape, and B allele (162) had negative effects with abovecharacters; For ETH225, C allele (138) had a positive relation with shoulders; ForBM1824, C allele (176) had negative effects with body diagonal length, heart girth,left-forelimb girth, withers, loin width, loin thickness, thigh muscularity, buttocksshape.