| This study was conducted to research the effects of double-low rapeseed meal and adding complex enzymes on the growth performance and feed utilization of the growing and finishing pigs, and to discuss the main mechanism though nutrient and amino acid apparent digestibility, the serum immune and antioxidant indicators, thyroid hormone and calcium, phosphorus, urea nitrogen level.This research concludes two experiments:Experiment 136 health growing pigs (Duroc×Landrace×Yorkshire) with an average initial body weight were randomly allocated to three groups by weigth and sex, each was replicated three times with four pigs per replicate. The control group was fed the corn-soybean meal (SBM) diet In the test group one (double-low rapeseed meal, DL-RSM group),8% double-low rapeseed meal was taken replacement of 7% SBM of the control group diet on the basis of the isonitrogen, isocalorie and balance of the digestible amino acid. In the test group 2 (DL-RSM+Enzymes group),0.03% complex enzymes (cellulase 10000IU/g, xylanase 6000IU/g, mannanase 5000IU/g, protease 12000IU/g) were supplemented on the basis of DL-RSM group'diet All pigs were given adequate diets and free access to water. The feeding experiment lasted for 32 days after 7 days of adaptation period.Experiment 236 health finishing pigs (DurocxLandracexYorkshire) were selected and arranged as experiment 1. The control group was fed the corn-soybean meal (SBM) diet In the test group one (double-low rapeseed meal, DL-RSM group),13% double-low rapeseed meal was taked replacement of 11% SBM of the control diet on the basis of the isonitrogen, isocalorie and balance of the digestible amino acid. In the test group 2 (DL-RSM+Enzymes group),0.03% complex enzymes (cellulase 10000IU/g, xylanase 6000IU/g, mannanase 5000IU/g, protease 12000IU/g) were supplemented on the basis of DL-RSM group'diet The feeding experiment lasted for 51 days after 7 days of adaptation period.After the rearing experiment began for two weeks, the fresh feces were collected and added H2SO4. The fecal samples, which were collected for 3 days, were mixed evenly, and then made into dried samples, which were kept at-4℃for further analysis. At the end of the trial, after weighing, the serum was collected and kept at-70℃for laboratory analysis.1. Results of the feeding experiment indicated that different treatment diets had no significant effects on the growth performance and feed utilization of the growing and finishing pigs (P>0.05). Compared with the control group, the daily weight gain and feed conversion efficiency of growing pigs in DL-RSM group had a decreasing trend, while supplement of the complex enzymes improved the aboved indicators (P>0.05).2. The results of digestive experiment showed that apparent nutrient and amino acids digestibility had no significant difference (P>0.05) between the control group and DL-RSM group, but these indicators were improved after adding the complex enzymes, the apparent EE, CF and TSAA digestibility in growing pigs increased by 14.64%(P<0.01), 21.61%(F<0.01) and 4.98%(P<0.05) respectively, meanwhile, the apparent DM and total phosphorus digestibility increased by 3.49%(P<0.05) and 32.53%(P<0.05), respectively.3. Results of hormone determination showed compared to DL-RSM group, adding the complex enzymes significantly increased the serum FT3 level of the growing pigs by 17.01%(P<0.01), while other indicators showed no significant difference (P>0.05) among these groups.4. Immune indicators showed that comparing with the control group, the serum IgA level was increased by 14.08%(P<0.01) in the growing pigs, the serum IgA, IgG, IgM, C3 and C4 levels decreased by 15.87%(P<0.01),12.40%(P<0.01),10.34%(P<0.05),20.55% (P<0.01) and 17.78%(P<0.01) in the finishing pigs of the DL-RSM group. Compared to the DL-RSM group, adding the complex enzymes increased the serum IgG, IgM, C3 and C4 levels of the growing pigs by 12.59%(P<0.01),20.59%(P<0.01),36.18%(P<0.01) and 24%(P<0.01), the serum IgA, IgG, IgM, C3 and C4 levels of the finishing pigs 37.74% (P<0.01),27.43%(P<0.01),28.85%(P<0.01),41.38%(P<0.01) and 32.43%(P<0.01), respectively.5. Antioxidant system function determination revealed that different treatment diets had no significant effects on serum antioxidant indicators in the growing and finishing pigs (P>0.05), but compared to the control group, the serum T-AOC, SOD and GSH-Px activity had an decreasing trend, and MDA content had an increasing trend in DL-RSM group (P>0.05), while adding enzymes had a certain degree of improving effects (P>0.05).6. Other serum indicators showed that different treatment diets had no significant effects on Ca, P, BUN level in the growing and finishing pigs, but compared to the control group, Ca, P content showed a decreasing trend and BUN level showed a increasing trend in DL-RSM group (P>0.05), the results above showed an improving trend after adding the complex enzymes (P>0.05). These results suggested that replacing 8% and 13% DI-RSM with 7% and 11% SBM in the growing and finishing pigs'diets, respectively, had no significant effects on apparent nutrient and amino acid digestibility, thyroid hormone secretion, body antioxidant capacity, growth performance and feed utilization effects were nearly consistent Supplementation of complex enzymes had positive effects and significantly declined the adverse effects of the DL-RSM on the immune function.
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