Cashmere Goats Izumo Gene Cloning And Tissue Expression

Mammalian fertilization process is a precise and orderly process of interaction between cells, the end result is that sperm-egg membrane fusion between the adoption of the role of a fertilized egg. At present, the scientists although the theory and through genomics proteomics technology found in the sperm surface fertilization associated with a significant number of molecules, but the sperm-egg fusion process is after the acrosome reaction carried out, in which sperm and egg plasma membrane must match the receptor binding, and this lead to what the molecular mechanism of sperm-egg fusion is unclear how it will operate. Has been obtained in these processes associated with sperm-egg fusion molecules, only recently discovered in the sperm of IGSF (immunoglobulin superfamily) new members of Izumo gene knockout mice after experiments prove that it is sperm-egg fusion necessary.Inner Mongolia White Cashmere goats for many years as a foster livestock in Inner Mongolia, is the main source of wool and cashmere, one of the research on the Izumo gene reported in this paper are first. Since the discovery of Izumo gene for understanding the mechanism of fertilization and goats have important guiding significance, in order to study in Inner Mongolia White Cashmere goats in the molecular mechanism of sperm-egg fusion, this study has been published under the GenBank, the nucleotide sequence of bovine Izumo, designed and synthesized goats Izumo gene amplification primers 1, experiment using RT-PCR (reverse transcription polymerase chain reaction) amplification of the Izumo gene sequences, and Izumo gene was cloned and sequenced. In this basis, to get some goats Izumo sequence as a template, using RACE (rapid amplification of cDNA ends) technology, the first expansion of the adult male testis Inner Mongolia Cashmere goats in Izumo gene sequences, and fishing took the gene CDS (coding region). Also this experiment was prepared digoxigenin labeled goat Izumo cDNA probe technology with the use of RNA Northern imprint on the Inner Mongolia White Cashmere goats Izumo RNA analysis, and thus the determination of total RNA, mRNA encoding Izumo gene expression of molecular size and abundance.1. By RT-PCR technique, the Izumo gene was 484 bp cDNA sequence, and the cattle Izumo gene homology as high as 98%.2. By RACE technology to obtain 1536 bp full-length sequence, fishing access to the 1035 bp of the Izumo gene coding sequence, the coding region encoding 344 amino acids.3. Through the goat testis and epididymis head, body, tail tissue Izumo gene mRNA in the RT-PCR identification of tissue expression, and mRNA of the gene in the Northern blotting results of testis and epididymis of the head, body, tail tissue show positive bands.4. This study further goats Izumo sperm-egg fusion in the process of gene function in the foundation.