Fraction And Preparation Of Extracellular Metabolites From Liquid Culture Agrocybe Chaxingu Huang And Their Antioxidant Characters

In this study, the water extracts of Camellia oleifera Abel branch powder and wheat bran were used in liquid medium for the submerged culture of Agrocybe chaxingu Huang. Three experiments were conducted, in which to compare the extracellular metabolites of A. chaxingu in polysaccharides and phenolic compounds from different culture medium and to study the techniques in the preparation for extracellular metabolites (Experiment 1); to evaluate the in vivo antioxidant effect of spray dried extracellular metabolites withβ-cyclodextrin (Experiment 2), freee dried extracellular polysaccharides and phenolic metabolites (Experiment 3), respectively, in mice. The growing performance, of mice and their viscera index were compared, the SOD and MDA content in serum were measured.The water extract of wheat bran (CK-WB), Camellia oleifera Abel branches (CK-YC) and the mixture of the water extract of wheat bran and Camellia oleifera Abel branches (WB-YC) were compared as three culture media. The extracellular polysaccharide and phenolic compounds in the supernatant of submerged culture was separated with ethanol precipitation, then freeze dried. The content of polysaccharides and phenolic, and their in vitro antioxidant characters were determined. The results showed that the total polysaccharides in supernatant of subermerged culture was higher in the group of CK-WB and WB-YC than in CK-YC (P <0.05), while the total phenolic content was higher in WB-YC group followed by CK-WB than in CK-YC group (P <0.05). The clerarance rate of hydroxyl radical in Fention system rated in the order: CK-WB> WB-YC> CK-YC (P <0.05).The extracellular metabolites of A. chaxingu were spray dried by use ofβ-cyclodextrin and were given to mice orally by gavage in different dosages. The results showed that the thymus index of mice was increased by giving spray dried submerged culture supernatant in all dosage level for CK-YC and WB-YC. In addition, for mice given 600-800mg/kgbw.d of spray dried supernatant of submerged culture, the SOD of mice serum was significantly higher in WB-YC than in CK-YC group (P <0.05).Further more, the effect of phenolic and polysaccharide metabolits on improving antioxidation capacity of mice in vivo was evaluated. Comparing with mice given with saline, the extracellular phenolic metabolites of A. chaxingu in WB-YC and CK-YC groups increased SOD activity in mice serum significantly (P <0.05). The increases in SOD activity were also observed in mice give by polysaccharide metabolits. Moreover, comparing with mice given with saline, the decrease of MDA content in mice serum was found from various groups, especially in polysaccharide group of WB-YC (P<0.05).