Studies On Establishment And Optimization Of Regeneration System In Jatropha Curcas L.

Jatropha curcas L. a kind of shrub or small tree in nettlespurge, is a member of the family Euphorbiaceae. It has been considered as a potential energy plant and has great advantage in pharmaceutical industry. In the paper, the culture condition was optimized, and an efficient regeneration system was established. The main results were as follows: 1. Optimization of the culture conditionThe most effective sterilization method of Jatropha curcas L. seeds was identified. The seeds were sterilized in 75% alcohol for 1 min, followed by in 0.1%HgCl2 for 20 min, then in 3%NaC10 for 30 min, and washed with sterile distilled water for five times. After sterilization the seeds were kept for 24 hours in sterile water. Removed the seed capsule and the aseptic seedlings were germinated from MS medium. Results showed that the pollution rate was 6.7%, and the germination rate was 97.8%. The best culture condition was following: cotyledons of 15 days seedlings were the best explants; cotyledons should be cutted to 1 cm×1 cm; the best light condition was 16h/d, and 40μmol m-2 s-1. 2. Establishment of high efficient regeneration system of Jatropha curcas L.The cotyledons of 15 days aseptic seedlings were used as explants of tissue culture. MS +6-BA 0.4 mg/L+IB A 0.5 mg/L+TDZ 0.1 mg/L was the most effective medium for callus induce. The browning rate was significantly decreased after 0.1 g/L active carborn was added, and the inductivity rate showed as 77.8%, was not decreased. The best medium of differentiation of adventitious buds from callus was MS+6-BA 0.8 mg/L+IB A, differentiation rate as 55.6%. We also achieved a protocol that the adventitious buds were immediacy differentiated without callus from cotyledons with 15 days aseptic seedlings. MS +6-BA 1.2 mg/L+IB A 0.05 mg/L was the best medium to induce buds, with inductivity rate as 35.5%; MS+6-BA 1.2 mg/L+IBA 0.05 mg/L+GA30.4 mg/L was the best medium to differentiate the buds, with the differentiation rate as 41.8%; MS+6-BA 0.1 mg/L+IBA 0.2 mg/L was vail for buds to elongate. The optimum rooting was MS+IBA0.1 mg/L+ NAA0.4mg/L, with rooting rate as 46.7%. The plantlets was transplanted in medium with soil and perlite with a ratio of 1:1, and the survival rate of transplanting were 78%.