| Salvia miltiorrhiza Bge. is a bulk herbs with high medicinal value and economic value in China. Now, there is a large market demand at home and abroad. In recent years, wether the varieties or the planting areas are all in the rapid expansion. The Chinese herbal medicine has become a major pillar industries in Anguo city of Hebei province. S. miltiorrhiza is the authentic herb in Anguo city of Hebei province and enjoyde the name of one of the eight drugs in Qi . However,it prone to low survival rate,pest disease and other issues in the cultivation process, resulting the yield and quality of S. miltiorrhiza in declining. AM fungi widely distributed in terrestrial ecosystems and has many beneficial effects on plant.It can promote plant growth and development , to improve plant disease resistance and stress tolerance. So, it has knowed as bio-fertilizer . It also could improve the soil physical and chemical properties, maintenance plant rhizosphere soil ecosystem virtuous circle. In this study, the symbiotic relationship of the S. miltiorrhiza and the AM fungi were used as an entry point, the community composition, the spatio-temporal distribution, species diversity and their relations with soil factors were investigated and analyzed. Wish can offer the material and basis to screening of AM fungi dominant species, use of mycorrhizal biotechnology to improve yield and quality of S. miltiorrhiza. Main research results are as follows:1 An abundant species resources of AM fungi was found in the rhizosphere of the S. miltiorrhiza. Isolation and purification of the mycorrhizal fungi led to the identification of 24 species belonged to 4 genera: 16 to the Glomus, 5 to the Acaulospora 2 to the Scutellospora and 1 to the Entrophospora.2 Ecological distribution of the diverse species is different: Glomus and Acaulospora in the three sampling sits are all have distribution, Scutellospora only occurred in Qicun, Entrophospora only occurred in Shatou. Some species of AM fungi only occurred in one samplingsites, such as Acaulospora.laevis, Scutellospora.calospor, Scutellospora.erythropa only occurred in Qicun. Glomus.geosporum only occurred in Zhongyaocaizhongzhijidi. Entrophospora. Infrequens and Glomus.multicaule only occurred in Shatou.3 S. miltiorrhiza can form well symbionts with AM fungi. The average colonization is up to 68.1%, and the average spore density reach 1714.9/100g soil. The roots of S. miltiorrhiza could be infencted by AM fungi and form a typical mycorrhizae, then growth form of vesicles and arbuscular. The mycorrhizal type is intermediate type.4 A distinct spatial and seasonal variation of AM fungi from the S. miltiorrhiza was found. Spore density and colonization in Zhongzhijidi were higher than in other two sampling sites. The greatest colonization and spore density levels were found in the second layers, between 10~20cm. October spore density and colonization was significantly higher than in July.5 The AM fungal species diversity and distribution in the three sites are different.The dominant species in three sites is not have a significant difference,scuh as Glomus.aggregatum is the common dominant species in three sites. However, there are differences in AM fungal species composition. There is a significant difference of the AM fungi composition between in Zhongzhijidi and Shatou. The AM fungi richness in Qicun is significant higher than in Zhongzhijidi and Shatou, but the evenness is the lowest. Shannon-Wiener indices at the three sites listed in order were: Shatou=Zhongzhijidi>Qicun.6 The AM fungi species diversity is closely related with soil factors. The available N and organic matter content in Qicun was higher than in other sites,but the spore density and the species richnness lower than other sites.The spore density and species richnness had a correlated negative with available N and organic.
|
PDF Full Text Request